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Invitrogen
Atrial Natriuretic Peptide (ANP) Competitive ELISA Kit
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产品规格
检测灵敏度
0.25 ng/mL
测定范围
0.741-180 ng/mL
检测样本类型/体积
{Plasma=50 µL, Supernatant=50 µL, Urine=12.5 µL}
手工操作时间
1 hr 20 min
总时间
1 hr 30 min
均相（无需洗涤）
No
批间差异
7.6%
批内差异
6.7%
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate
Standard
Assay Diluent concentrate
HRP-Conjugated Detection Antibody
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Assay Diluent concentrate
HRP-Conjugated Detection Antibody
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
运输条件
Wet ice
储存
-20°C
蛋白名称
ANP
均相（无需洗涤）
Chemical
检测试剂盒格式
Competitive ELISA
检测抗体结合物
HRP
标签或染料
HRP
关于本试剂盒
The Atrial Natriuretic Peptide (ANP) Competitive ELISA quantitates ANP in human plasma, urine or cell culture medium.
原理方法
The Atrial Natriuretic Peptide (ANP) Competitive ELISA research-use-only kit is designed to quantitatively measure ANP present in plasma, urine, and tissue culture media samples. This kit is not recommended for serum or plasma samples without extraction. An ANP standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture rabbit antibodies. An ANP-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of an antibody to ANP to each well. After a 1-hour incubation the plate is washed and substrate is added. The substrate reacts with the bound ANP-peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring at 450 nm.
严格验证
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
靶标信息
The protein encoded by this gene belongs to the natriuretic peptide family. Natriuretic peptides are implicated in the control of extracellular fluid volume and electrolyte homeostasis. This protein is synthesized as a large precursor (containing a signal peptide), which is processed to release a peptide from the N-terminus with similarity to vasoactive peptide, cardiodilatin, and another peptide from the C-terminus with natriuretic-diuretic activity. Mutations in this gene have been associated with atrial fibrillation familial type 6.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名 : ANP, Pronatriodilatin, urodilatin
