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Invitrogen
Basic Human PD-1 ELISA Kit
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产品规格
检测灵敏度
1.14 pg/mL
测定范围
2.34-150 pg/mL
检测样本类型/体积
{Supernatant=50 µL, Plasma=50 µL, Serum=50 µL}
手工操作时间
1 hr 20 min
总时间
3 hr 30 min
均相（无需洗涤）
No
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate
Standard
Sample Diluent
Assay Buffer concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Sample Diluent
Assay Buffer concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
运输条件
Wet ice
储存
2-8°C
蛋白名称
PD-1 (CD279)
均相（无需洗涤）
Human
检测试剂盒格式
Sandwich ELISA Kit
检测抗体结合物
Biotin
标签或染料
HRP
关于本试剂盒
Kit Overview
The Human PD-1 ELISA kit enables the quantification of Human PD-1 in various biological samples, including human serum, plasma, buffered solutions, and cell culture medium. This assay is specifically designed to exclusively detect both natural and recombinant forms of Human PD-1.
Principle of the assay
The Human PD-1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) follows a unique approach to measure the amount of the target molecule. It utilizes a matched pair of antibodies to capture the target and form a sandwich complex. The first antibody is pre-coated on the microplate wells, where samples, standards, or controls are added and bind to the immobilized antibody. The second antibody, acting as the detector, is then introduced, followed by the addition of a substrate solution that generates a measurable signal upon interaction with the enzyme-antibody-target complex. The intensity of this signal directly correlates to the concentration of the target present in the original specimen.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
靶标信息
Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antig en-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of the immune response in the absence of "danger signals" such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PD1-null mice who exhibit hyperactivated immune systems and autoimmune diseases. Despite its predicted molecular weight, PD-1 often migrates at higher molecular weight in SDS-PAGE.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
基因别名 : CD279, hPD-1, hPD-l, hSLE1, PD-1, PD1, PDCD1, SLEB2
基因ID : (Human) 5133
基因符号 : PDCD1
蛋白别名 : CD279, hPD1, Protein PD1, sCD279, soluble CD279, programmed cell death 1 protein, Programmed cell death protein 1, Protein PD-1, systemic lupus erythematosus susceptibility 2
UniProt ID (Human) Q15116
