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Invitrogen
Basic Human PDGF-BB ELISA Kit
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产品规格
检测灵敏度
4.6 pg/mL
测定范围
31.3-2,000 pg/mL
检测样本类型/体积
{Supernatant=20 µL, Serum=20 µL, Plasma=20 µL}
手工操作时间
1 hr 20 min
总时间
3 hr 30 min
均相（无需洗涤）
No
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate
Standard
Sample Diluent
Assay Buffer concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Sample Diluent
Assay Buffer concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
运输条件
Wet ice
储存
2-8°C
蛋白名称
PDGF-BB
均相（无需洗涤）
Human
检测试剂盒格式
Sandwich ELISA Kit
检测抗体结合物
Biotin
标签或染料
HRP
关于本试剂盒
Kit Overview
The Human PDGF-BB ELISA kit enables the quantification of Human PDGF-BB in various biological samples, including human serum, plasma, buffered solutions, and cell culture medium. This assay is specifically designed to exclusively detect both natural and recombinant forms of Human PDGF-BB.
Principle of the assay
The Human PDGF-BB solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) follows a unique approach to measure the amount of the target molecule. It utilizes a matched pair of antibodies to capture the target and form a sandwich complex. The first antibody is pre-coated on the microplate wells, where samples, standards, or controls are added and bind to the immobilized antibody. The second antibody, acting as the detector, is then introduced, followed by the addition of a substrate solution that generates a measurable signal upon interaction with the enzyme-antibody-target complex. The intensity of this signal directly correlates to the concentration of the target present in the original specimen.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
靶标信息
The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 7, at sites where this gene and that for COL1A1 are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans resulting from unregulated expression of growth factor. Two alternatively spliced transcript variants encoding different isoforms have been identified for this gene.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
基因别名 : c-sis, IBGC5, PDGF-2, PDGF2, PDGFB, SIS, SSV
基因ID : (Human) 5155
基因符号 : PDGFB
蛋白别名 : becaplermin, FLJ12858, H-PDGF-BB, PDGF subunit B, PDGF, B chain, PDGF-2, platelet-derived growth factor 2, Platelet-derived growth factor B chain, Platelet-derived growth factor beta polypeptide, platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog), Platelet-derived growth factor subunit B, platelet-derived growth factor, beta polypeptide (oncogene SIS), Proto-oncogene c-Sis
UniProt ID (Human) P01127
