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Invitrogen
Basic Mouse GM-CSF ELISA Kit
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产品规格
检测灵敏度
2 pg/mL
测定范围
3.9-250 pg/mL
检测样本类型/体积
{Supernatant=50 µL, Serum=50 µL}
手工操作时间
1 hr 20 min
总时间
3 hr 30 min
均相（无需洗涤）
No
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate
Standard
Sample Diluent
Assay Buffer concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Sample Diluent
Assay Buffer concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
运输条件
Wet ice
储存
2-8°C
蛋白名称
GM-CSF
均相（无需洗涤）
Mouse
检测试剂盒格式
Sandwich ELISA Kit
检测抗体结合物
Biotin
标签或染料
HRP
关于本试剂盒
Kit Overview
The Mouse GM-CSF ELISA kit enables the quantification of Mouse GM-CSF in various biological samples, including human serum, plasma, buffered solutions, and cell culture medium. This assay is specifically designed to exclusively detect both natural and recombinant forms of Mouse GM-CSF.
Principle of the assay
The Mouse GM-CSF solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) follows a unique approach to measure the amount of the target molecule. It utilizes a matched pair of antibodies to capture the target and form a sandwich complex. The first antibody is pre-coated on the microplate wells, where samples, standards, or controls are added and bind to the immobilized antibody. The second antibody, acting as the detector, is then introduced, followed by the addition of a substrate solution that generates a measurable signal upon interaction with the enzyme-antibody-target complex. The intensity of this signal directly correlates to the concentration of the target present in the original specimen.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
靶标信息
The GM-CSF gene encodes for a cytokine that regulates the production, differentiation, and function of macrophages and granulocytes. The active form of the protein exists as a homodimer in the extracellular space. This gene is located in a cluster of related genes at the chromosome region 5q31, which has been associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include interleukins 4, 5, and 13. The gene is involved in promoting tissue inflammation. Elevated levels of cytokines, including the one produced by this gene, have been observed in SARS-CoV-2 infected patients with acute respiratory distress syndrome. Mice that lack this gene or its receptor have been shown to develop pulmonary alveolar proteinosis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
基因别名 : Csf2, Csfgm, Gm-CSf, GMCSF, MGI-IGM
基因ID : (Mouse) 12981
基因符号 : Csf2
蛋白别名 : Colony-stimulating factor, CSF, GM-CSF, granulocyte-macrophage colony stimulating factor 2, Granulocyte-macrophage colony-stimulating factor, MGC138897, MGC151255, MGC151257, M-GM-CSF, granulocyte macrophage colony stimulating factor, MGC131935, put. GM-CSF
UniProt ID (Mouse) P01587
