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Invitrogen
Human Adiponectin Rapid ELISA Kit
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产品规格
检测灵敏度
1.88 ng/mL
测定范围
3.13-200 ng/mL
检测样本类型/体积
{Plasma=50 µL, Serum=50 µL}
手工操作时间
15 min
总时间
1 hr 30 min
均相（无需洗涤）
No
批间差异
<10%
批内差异
<10%
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate, Standard, HRP Conjugate, Standard & Sample Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
运输条件
Wet ice
储存
Refer to product documentation for component specific storage temperature.
蛋白名称
Adiponectin
均相（无需洗涤）
Human
检测试剂盒格式
Rapid ELISA Kit
检测抗体结合物
HRP
标签或染料
Unconjugated
关于本试剂盒
The Human ADP/Acrp30 (Adiponectin) ELISA quantitates ADP in serum and plasma.
原理方法
The Human Adiponectin (ADP/Acrp30) solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the concentration of Adiponectin (ADP/Acrp30) in biological samples. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are added into these wells and bind to the immobilized (capture) antibody.
The sandwich complex is formed by the addition of a second (HRP-linked) detection antibody specific to Adiponectin (ADP/Acrp30). Excess reagents are washed from the plate.
A substrate solution is added that reacts with the enzyme-antibody-target complex to produce a measurable signal. The enzyme-substrate reaction is terminated by the addition of stop solution. The intensity of the signal, measured spectrophotometrically at 450 ± 2 nm, is directly proportional to the concentration of Adiponectin (ADP/Acrp30) present in the original specimen.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
靶标信息
Adiponectin is a protein hormone secreted exclusively by adipocytes that regulates the metabolism of lipids and glucose. Adiponectin has been shown to play a role in various physiological processes including homeostasis and obesity. Decreased levels of adiponectin are associated with obese individuals as well as insulin resistance and hyperinsulinemia (type 2 diabetes). In mice adiponectin has been shown to improve insulin signaling, thus ameliorating insulin resistance. Through activation of AMP kinase and PPAR-alpha, adiponectin can increase fatty acid transport, oxidation, and dissipation in skeletal muscle, leading to a reduction of intramyocellular lipid levels and thus improved insulin signaling.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
基因别名 : ACDC, ACRP30, ADIPOQ, ADIPQTL1, ADPN, APM-1, APM1, GBP28
基因ID : (Human) 9370
基因符号 : ADIPOQ
蛋白别名 : 30 kDa adipocyte complement-related protein, ACRP30, Adipocyte complement-related 30 kDa protein, Adipocyte, C1q and collagen domain-containing protein, Adiponectin, Adipose most abundant gene transcript 1 protein, adipose specific collagen-like factor, ADPIOQ, apM-1, Gelatin-binding protein, gelatin-binding protein 28, unnamed protein product
UniProt ID (Human) Q15848
