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Invitrogen
Human Anti-IFN alpha Antibody ELISA Kit
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产品规格
检测灵敏度
0.6 ng/mL
测定范围
3.1-200 ng/mL
检测样本类型/体积
{Serum=20 µL, Plasma=20 µL, Urine=20 µL}
手工操作时间
1 hr 20 min
总时间
3 hr 30 min
均相（无需洗涤）
No
批间差异
6.3%
批内差异
8%
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated microwell plate
Standard
Streptavidin-HRP
Sample Diluent
Wash Buffer Concentrate
Assay Buffer concentrate
Stop Solution
Substrate Solution
Adhesive Plate Covers
Standard
Streptavidin-HRP
Sample Diluent
Wash Buffer Concentrate
Assay Buffer concentrate
Stop Solution
Substrate Solution
Adhesive Plate Covers
运输条件
Wet ice
储存
2-8°C
蛋白名称
Anti-IFN alpha Antibody
均相（无需洗涤）
Human
检测试剂盒格式
Sandwich ELISA Kit
检测抗体结合物
Biotin
标签或染料
HRP
关于本试剂盒
This specific product detects antibodies directed against interferon alpha. The Human Interferon alpha Antibody (Hu IFN alpha) ELISA quantitates Hu IFN alpha in human serum, plasma or urine samples. The assay will exclusively recognize both natural and recombinant Hu IFN alpha.
原理方法
The Human IFN alpha solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
靶标信息
This specific product detects antibodies directed against interferon alpha. IFN alpha (Interferon alpha) is a Type I interferon belonging to a family of structurally related, species-specific proteins exclusive to vertebrates. The interaction of IFN-alpha with its receptor component result in the activation of a number of signaling pathways which are mainly involved in innate immune response against viral infection. In humans, the gene for IFN alpha is located on on chromosome 9. The type I interferons consist of 14 different alpha isoforms (subtypes with slightly different specificities), and single beta, omega, epsilon and kappa isoforms. Homologous molecules are found in many species, including rats and mice (and most mammals), and have been identified in birds, reptiles and fish species. IFN alpha is produced primarily by plasmacytoid dendritic cells, is a potent component of the anti-viral innate immune response and modulates adaptive immunity. Diseases associated with the dysfunction of IFN alpha include Hepatitis C and Hepatitis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名 : Anti interferon alpha, Anti-IFN, Anti-IFN alpha, Anti-IFNa
