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Invitrogen
Human E-cadherin Rapid ELISA Kit
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产品规格
检测灵敏度
46.88 pg/mL
测定范围
78.13-5000 pg/mL
检测样本类型/体积
{Plasma=50 µL, Serum=50 µL}
手工操作时间
15 min
总时间
1 hr 30 min
均相（无需洗涤）
No
批间差异
<10%
批内差异
<10%
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate, Standard, HRP Conjugate, Standard & Sample Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
运输条件
Wet ice
储存
Refer to product documentation for component specific storage temperature.
蛋白名称
E-cadherin
均相（无需洗涤）
Human
检测试剂盒格式
Rapid ELISA Kit
检测抗体结合物
HRP
标签或染料
Unconjugated
关于本试剂盒
The Human E-Cad (E-Cadherin) ELISA quantitates E-Cad in serum, plasma and other biological fluids.
原理方法
The Human E-Cadherin (E-Cad) solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the concentration of E-Cadherin (E-Cad) in biological samples. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are added into these wells and bind to the immobilized (capture) antibody.
The sandwich complex is formed by the addition of a second (HRP-linked) detection antibody specific to E-Cadherin (E-Cad). Excess reagents are washed from the plate.
A substrate solution is added that reacts with the enzyme-antibody-target complex to produce a measurable signal. The enzyme-substrate reaction is terminated by the addition of stop solution. The intensity of the signal, measured spectrophotometrically at 450 ± 2 nm, is directly proportional to the concentration of E-Cadherin (E-Cad) present in the original specimen.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
靶标信息
E-Cadherin (epithelial cadherin) is a classical cadherin from the cadherin (alcium dependent adhesion protein) superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. E-cadherin plays a central role in the growth and development of cells by controlling tissue architecture, and maintenance of tissue integrity. In humans, E-cadherin is encoded by the CDH1 gene present on chromosome 16. Studies have demonstrated that reduction and/or loss of E-cadherin expression in carcinomas correlates positively with the potential of these tumors for invasion and metastasis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
基因别名 : Arc-1, BCDS1, CD324, CDH1, CDHE, ECAD, LCAM, UVO
基因ID : (Human) 999
基因符号 : CDH1
蛋白别名 : cadherin 1, E-cadherin (epithelial), type 1, cadherin e, cadherin-E, Ecadherin, Cadherin-1, calcium-dependent adhesion protein, epithelial, CAM 120/80, CD324, cell-CAM 120/80, E-cadherin, E-cadherin 1, epididymis secretory sperm binding protein, Epithelial cadherin, unnamed protein product, Uvomorulin
UniProt ID (Human) P12830
