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Invitrogen
Human FABP-3 Rapid ELISA Kit
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产品规格
检测灵敏度
0.19 ng/mL
测定范围
0.31-20 ng/mL
检测样本类型/体积
{Serum=50 µL, Plasma=50 µL}
手工操作时间
15 min
总时间
1 hr 30 min
均相（无需洗涤）
No
批间差异
<10%
批内差异
<10%
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate, Standard, HRP Conjugate, Standard & Sample Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
运输条件
Wet ice
储存
Refer to product documentation for component specific storage temperature.
蛋白名称
FABP3
均相（无需洗涤）
Human
检测试剂盒格式
Rapid ELISA Kit
检测抗体结合物
HRP
标签或染料
Unconjugated
关于本试剂盒
The Human FABP3 (Fatty Acid Binding Protein 3, Muscle and Heart) ELISA quantitates FABP3 in serum and plasma.
原理方法
The Human Fatty Acid Binding Protein 3, Muscle and Heart (FABP3) solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the concentration of Fatty Acid Binding Protein 3, Muscle and Heart (FABP3) in biological samples. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are added into these wells and bind to the immobilized (capture) antibody.
The sandwich complex is formed by the addition of a second (HRP-linked) detection antibody specific to Fatty Acid Binding Protein 3, Muscle and Heart (FABP3). Excess reagents are washed from the plate.
A substrate solution is added that reacts with the enzyme-antibody-target complex to produce a measurable signal. The enzyme-substrate reaction is terminated by the addition of stop solution. The intensity of the signal, measured spectrophotometrically at 450 ± 2 nm, is directly proportional to the concentration of Fatty Acid Binding Protein 3, Muscle and Heart (FABP3) present in the original specimen.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
靶标信息
Fatty acid binding proteins (FABP) are small (approximately 13-14 kDa) intracellular proteins with a high degree of tissue specificity. FABPs are a class of cytoplasmic proteins that bind long chain fatty acids. They are abundantly present in various cell types and seem to play an important role in the intracellular utilization of fatty acids. There are at least six distinct types of FABP, each showing a specific pattern of tissue expression. FABP leaks, due to its small size, rapidly out of is chemically damaged dying cells leading to a rise in serum levels. Liver FABP is a sensitive marker for cell damage of liver cells in vitro and in vivo. Is chemically damaged tissues are characterized histologically by absence (or low presence) of FABP facilitating recognition of such areas. Next to this L FABP is a marker for rapid hepatocyte lysis in vitro (as for example in toxicology assays) and for detection of liver damage during and after transplantation.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
基因别名 : FABP11, FABP3, H-FABP, M-FABP, MDGI, O-FABP
基因ID : (Human) 2170
基因符号 : FABP3
蛋白别名 : 422 protein, Cardiac Fatty Acid Binding Protein, FABP 3, Fatty acid binding protein 11 (FABP11), Fatty acid binding protein heart, Muscle O-FABP, Mylein protein P2 homolog / adopocyte, heart FABP, Heart type fatty acid binding protein, M FABP, Mammary derived growth inhibitor, muscle and heart (mammary derived growth inhibitor), Muscle fatty acid binding protein, epididymis secretory sperm binding protein, FABP3, fatty acid binding protein 11, fatty acid binding protein 3, muscle and heart, Fatty acid-binding protein 3, Fatty acid-binding protein, heart, H-FABP, Heart-type fatty acid-binding protein, M-FABP, Mammary-derived growth inhibitor, MDGI, Muscle fatty acid-binding protein, unnamed protein product
UniProt ID (Human) P05413
