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Invitrogen
Human HLA-DR ELISA Kit
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Product Specifications
Analytical sensitivity
1.76 ng/mL
Assay range
1.76-500 ng /mL
Sample type/volume
Plasma
100 µL
Supernatant
100 µL
Serum
100 µL
Hands-on time
1 hr 20 min
Time-to-result
4 hr 45 min
Homogenous (no wash)
No
Interassay CV
12%
Intraassay CV
10%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated microwell plate
Standard
Biotinylated Detection Antibody
Streptavidin-HRP
Sample Diluent
Wash Buffer Concentrate
Assay Buffer concentrate
Stop Solution
Substrate Solution
Adhesive Plate Covers
Standard
Biotinylated Detection Antibody
Streptavidin-HRP
Sample Diluent
Wash Buffer Concentrate
Assay Buffer concentrate
Stop Solution
Substrate Solution
Adhesive Plate Covers
Shipping conditions
Wet ice
Storage
-20°C
Protein name
HLA-DR
Species (tested)
Human
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
Human HLA-DR ELISA Kit is an enzyme-linked immunosorbent assay for quantitative detection of human HLA-DR.
Target information
HLA-DR, like other MHC class II molecules, is a transmembrane glycoprotein composed of a 36 kDa alpha chain (DRA) and 27 kDa beta chain (DRB). The alpha chain gene contains 5 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. DRA does not have polymorphisms in the peptide binding part and acts as the sole alpha chain for DRB1, DRB3, DRB4 and DRB5. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Hundreds of DRB1 alleles have been described and typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. HLA-DR is expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, thymic epithelial cells and activated T lymphocytes. Three loci, DR, DQ and DP, encode the major expressed products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides, present them to T-helper cells, and have a critical role in the initiation of the immune response.
HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : CD74, CLIP, DHLAG, DR4, DRB1, DRB3, DRB4, DRB5, HLA-DPB1, HLA-DR1B, HLA-DR3B, HLA-DR4B, HLA-DRA, HLA-DRA1, HLA-DRB, HLA-DRB1, HLA-DRB3, HLA-DRB3*, HLA-DRB4, HLA-DRB4*, HLA-DRB5, HLA-DRB5*, HLADG, Ia-GAMMA, II, p33, SS1
Gene ID : (Human) 972, (Human) 3122, (Human) 3123, (Human) 3125, (Human) 3126, (Human) 3127
Gene symbol : CD74, HLA-DRA, HLA-DRB1, HLA-DRB3, HLA-DRB4, HLA-DRB5
Protein Aliases : CD74, CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated), CD74 antigen, DASS-397D15.1, FLJ51114, FLJ75017, FLJ76359, XXbac-BPG161M6.1, CD74 molecule, major histocompatibility complex, class II invariant chain, cell surface glycoprotein, integral membrane protein, class II HLA histocompatibility antigen, class II MHC-associated invariant chain peptide, DR beta-5, DR2-beta-2, DR7 beta-chain glycoprotein, DRB1, DRB52, DRw53 beta-chain glycoprotein, Dw2, ERLA-005, gamma chain of class II antigens, histocompatibility antigen HLA-DR alpha, HLA class II histocompatibility antigen gamma chain, HLA class II histocompatibility antigen, DR alpha chain, DR beta 3 chain, DR beta 4 chain, DR beta 5 chain, DR-1 beta chain, DR-5 beta chain, DRB1 beta chain, HLA DRB3, HLA-DR alpha-chain, hla-dr antigen alpha chain, hla-dr antigen heavy chain, HLA-DR antigens-associated invariant chain, HLA-DR-alpha chain precursor, HLA-DR-beta chain, HLA-DR-gamma, HLA-DR11-14N allele, HLA-DRA, HLA-DRB1, human leucocyte antigen, human leucocyte antigen DRB1, human leucocyte antigen DRB3, Human leukocyte antigen DRB1, Ia antigen-associated invariant chain, Ia-associated invariant chain, Ii, leukocyte antigen, lymphocyte antigen DRB1, class II, DRB3, MHC class II antigen, MHC class II antigen DR beta 3 chain, MHC class II antigen DRA, MHC class II antigen DRB3, MHC class II antigen DRB4, MHC class II antigen DRB5, MHC class II DR beta chain, MHC class II HLA-DR beta 1 chain, MHC class II HLA-DR beta 3 chain, MHC class II HLA-DR4 protein, MHC class II HLA-DR4/w53 protein, MHC class II protein, MHC DR beta protein precursor, MHC DR-beta, MHC DR7-beta-1 chain, MHC DR7-beta-2 chain, MHC HLA DR5 cell surface glycoprotein beta chain precursor, MHC HLA-DR gamma chain, MHC HLA-DR-beta chain, MHC HLA-DR-beta chain precursor, MHC HLA-DR-beta chain precursor old gene name 'HLA-DRA1', MHC HLA-DR-beta surface glycoprotein, MHC HLA-DR-beta-1 chain, MHC HLA-DR-beta-1 precursor, MHC HLA-DR-beta-2 precursor, MHC HLA-DR1-beta, MHC HLA-DR2 (non-Dw2/non-Dw12)b glycoprotein beta-chain, MHC HLA-DR2(Dw12)a glycoprotein beta-chain, MHC HLA-DR2(Dw12)b glycoprotein beta-chain, MHC HLA-DR2(Dw2)a glycoprotein beta-chain, MHC HLA-DR2(Dw2)b glycoprotein beta-chain, MHC HLA-DR2(non-Dw2/non-Dw12)a glycoprotein beta-chain, MHC HLA-DRw10-beta-2 protein precursor, unnamed protein product
UniProt ID (Human) P04233, (Human) P01903, (Human) P01911, (Human) P79483, (Human) P13762, (Human) Q30154
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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