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Invitrogen
Human HTRA1 Rapid ELISA Kit
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产品规格
检测灵敏度
0.19 ng/mL
测定范围
0.31-20 ng/mL
检测样本类型/体积
{Serum=50 µL, Plasma=50 µL}
手工操作时间
15 min
总时间
1 hr 30 min
均相（无需洗涤）
No
批间差异
<10%
批内差异
<10%
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate, Standard, HRP Conjugate, Standard & Sample Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
运输条件
Wet ice
储存
Refer to product documentation for component specific storage temperature.
蛋白名称
HTRA1
均相（无需洗涤）
Human
检测试剂盒格式
Rapid ELISA Kit
检测抗体结合物
HRP
标签或染料
Unconjugated
关于本试剂盒
The Human HTRA1 (HtrA Serine Peptidase 1) ELISA quantitates HTRA1 in serum and plasma.
原理方法
The Human HtrA Serine Peptidase 1 (HTRA1) solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the concentration of HtrA Serine Peptidase 1 (HTRA1) in biological samples. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are added into these wells and bind to the immobilized (capture) antibody.
The sandwich complex is formed by the addition of a second (HRP-linked) detection antibody specific to HtrA Serine Peptidase 1 (HTRA1). Excess reagents are washed from the plate.
A substrate solution is added that reacts with the enzyme-antibody-target complex to produce a measurable signal. The enzyme-substrate reaction is terminated by the addition of stop solution. The intensity of the signal, measured spectrophotometrically at 450 ± 2 nm, is directly proportional to the concentration of HtrA Serine Peptidase 1 (HTRA1) present in the original specimen.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
靶标信息
HtrA1, a 51KDa protein, is a member of highly conserved mammalian HtrA serine protease family. It is a human homolog of bacterial HtrA and consists of a secretory signal sequence, an IGFBP-binding domain and a Kazal-type serine-protease inhibitor domain at the N-terminus and a highly conserved trypsin-like serine/protease domain and a PDZ domain at the C-terminus. It plays a major role in the regulation of cell growth. HtrA1 inhibits signaling mediated by TGF-beta family members and regulates many physiological processes. HtrA1 is up-regulated in placenta during pregnancy and plays an important role in implantation and placental physiology. It regulates the availability of IGFs by cleaving IGF-binding proteins. It acts as a tumor suppressor and is down-regulated in ovarian cancers and melanomas. Its role has been implicated in osteoarthritis suggesting a therapeutic intervention. Northern blot analysis detected high expression in placenta.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
基因别名 : ARMD7, CADASIL2, CARASIL, HTRA, HTRA1, L56, ORF480, PRSS11
基因ID : (Human) 5654
基因符号 : HTRA1
蛋白别名 : High-temperature requirement A serine peptidase 1, IGFBP5-protease, L56, protease, serine, 11 (IGF binding), Serine protease 11, Serine protease HTRA1, similar to bacterial htrA and degP, unnamed protein product
UniProt ID (Human) Q92743
