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Invitrogen
Human HTRA1 Rapid ELISA Kit
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Product Specifications
Analytical sensitivity
0.19 ng/mL
Assay range
0.31-20 ng/mL
Sample type/volume
Serum
50 µL
Plasma
50 µL
Hands-on time
15 min
Time-to-result
1 hr 30 min
Homogenous (no wash)
No
Interassay CV
<10%
Intraassay CV
<10%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate, Standard, HRP Conjugate, Standard & Sample Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
Shipping conditions
Wet ice
Storage
Refer to product documentation for component specific storage temperature.
Protein name
HTRA1
Species (tested)
Human
Detector antibody conjugate
HRP
Label or dye
Unconjugated
About This Kit
The Human HTRA1 (HtrA Serine Peptidase 1) ELISA quantitates HTRA1 in serum and plasma.
Principle of the method
The Human HtrA Serine Peptidase 1 (HTRA1) solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the concentration of HtrA Serine Peptidase 1 (HTRA1) in biological samples. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are added into these wells and bind to the immobilized (capture) antibody.
The sandwich complex is formed by the addition of a second (HRP-linked) detection antibody specific to HtrA Serine Peptidase 1 (HTRA1). Excess reagents are washed from the plate.
A substrate solution is added that reacts with the enzyme-antibody-target complex to produce a measurable signal. The enzyme-substrate reaction is terminated by the addition of stop solution. The intensity of the signal, measured spectrophotometrically at 450 ± 2 nm, is directly proportional to the concentration of HtrA Serine Peptidase 1 (HTRA1) present in the original specimen.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Target information
HtrA1, a 51KDa protein, is a member of highly conserved mammalian HtrA serine protease family. It is a human homolog of bacterial HtrA and consists of a secretory signal sequence, an IGFBP-binding domain and a Kazal-type serine-protease inhibitor domain at the N-terminus and a highly conserved trypsin-like serine/protease domain and a PDZ domain at the C-terminus. It plays a major role in the regulation of cell growth. HtrA1 inhibits signaling mediated by TGF-beta family members and regulates many physiological processes. HtrA1 is up-regulated in placenta during pregnancy and plays an important role in implantation and placental physiology. It regulates the availability of IGFs by cleaving IGF-binding proteins. It acts as a tumor suppressor and is down-regulated in ovarian cancers and melanomas. Its role has been implicated in osteoarthritis suggesting a therapeutic intervention. Northern blot analysis detected high expression in placenta.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : ARMD7, CADASIL2, CARASIL, CARASIL2, HtrA, L56, ORF480, PRSS11
Gene ID : (Human) 5654
Gene symbol : HTRA1
Protein Aliases : high-temperature requirement A serine peptidase 1, IGFBP5-protease, protease, serine, 11 (IGF binding), similar to bacterial htrA and degP, unnamed protein product
UniProt ID (Human) Q92743
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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