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Invitrogen
Human ICAM-1 Rapid ELISA Kit
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Product Specifications
Analytical sensitivity
0.15 ng/mL
Assay range
0.25-16 ng/mL
Sample type/volume
Serum
50 µL
Plasma
50 µL
Hands-on time
15 min
Time-to-result
1 hr 30 min
Homogenous (no wash)
No
Interassay CV
<10%
Intraassay CV
<10%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate, Standard, HRP Conjugate, Standard & Sample Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
Shipping conditions
Wet ice
Storage
Refer to product documentation for component specific storage temperature.
Protein name
ICAM-1 (CD54)
Species (tested)
Human
Assay kit format
Rapid ELISA Kit
Detector antibody conjugate
HRP
Label or dye
Unconjugated
About This Kit
The Human ICAM-1/CD54 (intercellular adhesion molecule 1) ELISA quantitates ICAM-1 in serum, plasma and other biological fluids.
Principle of the method
The Human intercellular adhesion molecule 1 (ICAM-1/CD54) solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the concentration of intercellular adhesion molecule 1 (ICAM-1/CD54) in biological samples. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are added into these wells and bind to the immobilized (capture) antibody.
The sandwich complex is formed by the addition of a second (HRP-linked) detection antibody specific to intercellular adhesion molecule 1 (ICAM-1/CD54). Excess reagents are washed from the plate.
A substrate solution is added that reacts with the enzyme-antibody-target complex to produce a measurable signal. The enzyme-substrate reaction is terminated by the addition of stop solution. The intensity of the signal, measured spectrophotometrically at 450 ± 2 nm, is directly proportional to the concentration of intercellular adhesion molecule 1 (ICAM-1/CD54) present in the original specimen.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Target information
ICAM-1 (CD54) is an 85-110 kDa single-chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. ICAM-1 has 7 potential N-linked glycosylation sites and shares considerable amino acid sequence homology with ICAM-3 (CD50) and ICAM-2 (CD102). ICAM-1 binds to integrins of type CD11a/CD18 (leukocyte adhesion molecule, LFA-1), or CD11b/CD18 (Mac-1) and is exploited by Rhinovirus as a receptor. ICAM-1 is expressed by activated endothelial cells and detected on epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils, with lower levels that increase upon inflammation. ICAM-1 is also detected in some carcinoma and melanoma cells. Soluble ICAM-1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : BB2, CD54, ICAM1, P3.58
Gene ID : (Human) 3383
Gene symbol : ICAM1
Protein Aliases : CD54, cell surface glycoprotein P3.58, epididymis secretory sperm binding protein, Human rhinovirus receptor, Ly 47, MALA2, sCD54, soluble CD54, Surface antigen of activated B cells, ICAM-1, ICAM-1 soluble variant, Intercellular adhesion molecule 1, intercellular adhesion molecule 1 (CD54), human rhinovirus receptor, intercellular adhesion molecule-1 precursor, Major group rhinovirus receptor, major group rhinovirus receptor precusor, unnamed protein product
UniProt ID (Human) P05362
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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