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Invitrogen
Human L-Selectin/CD62L (Soluble) Instant ELISA™ Kit
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产品规格
检测灵敏度
0.19 ng/mL
测定范围
0.39-25.0 ng/mL
检测样本类型/体积
{Amniotic Fluid=50 µL, Plasma=10 µL, Serum=10 µL, Supernatant=50 µL}
手工操作时间
15 min
总时间
2 hr 10 min
均相（无需洗涤）
No
批间差异
10%
批内差异
9.2%
检测仪器
Absorbance Microplate Reader
产品规格
128 Tests
产品成分
Pre-coated 96 well Microplate
Detection Antibody
Anti-IgG-HRP
Sample Diluent, lyophilized
Standard (8-well strips x 4)
Wash Buffer Concentrate
Substrate Solution
Sample Diluent
Stop Solution
Lysis Buffer 10x
Adhesive Plate Covers
Detection Antibody
Anti-IgG-HRP
Sample Diluent, lyophilized
Standard (8-well strips x 4)
Wash Buffer Concentrate
Substrate Solution
Sample Diluent
Stop Solution
Lysis Buffer 10x
Adhesive Plate Covers
运输条件
Wet ice
储存
-20°C
蛋白名称
L-Selectin (CD62L)
Target site-specificity
Target sub-specificity
Soluble
检测试剂盒格式
Instant ELISA Kit
检测抗体结合物
HRP
标签或染料
HRP
关于本试剂盒
The Human Soluble L-Selectin/CD62L (Hu sCD62L) ELISA quantitates Hu sCD62L in human serum, plasma, cell culture supernatants, or amniotic fluid. The assay will exclusively recognize both natural and recombinant Hu sCD62L.
原理方法
The Human sCD62L solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
严格验证
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
靶标信息
The human CD62L is a 74-95 kDa glycoprotein member of the selectin family of adhesion receptors. L-Selectin is comprised of an aminoterminal C-type lectin binding domain, an epidermal growth factor-like domain, two short consensus repeat (SCR) sequences homologous to those found in complement binding proteins, a short spacer region, a transmembrane region and a short cytoplasmic region. Human CD62L (L-Selectin) is constitutively expressed on all classes of leukocytes including lymphocytes (except a substantial population of memory T cells), monocytes and polymorphonuclear cells.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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