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Invitrogen
Human MMP3 Matched Antibody Pair
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产品规格
检测灵敏度
<1.25 ng/mL
测定范围
1.25-20 ng/mL
检测样本类型/体积
{Plasma=100 µL, Serum=100 µL, Supernatant=100 µL}
手工操作时间
1 hr 30 min
总时间
24 hr
均相（无需洗涤）
No
检测仪器
Absorbance Microplate Reader
产品规格
10 x 96 Tests
产品成分
Capture (coating) Antibody
Detection Antibody
Standard
Streptavidin-HRP
Detection Antibody
Standard
Streptavidin-HRP
运输条件
Wet ice
储存
2-8°C
蛋白名称
MMP3
蛋白亚型
Proteases
均相（无需洗涤）
Human
检测试剂盒格式
Matched Antibody Pair
检测抗体结合物
Biotin
标签或染料
HRP
关于本试剂盒
The Human MMP3 Antibody Pair Kit comes with pre-matched antibody pairs, standards, and streptavidin-HRP to develop your own enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human MMP3. Buffer reagents needed to complete the ELISA reaction are sold separate as a Buffer Kit for Antibody Pairs (Catalog No. CNB0011). The Assay Buffer included in this Buffer Kit can be used as a blocking reagent for ELISA plates as well as a diluent for ELISA standards and samples, detection antibody, and HRP conjugate.
原理方法
ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
靶标信息
MMP3 (stromelysin-1) belongs to the peptidase M10A subfamily. It is secreted in its pro-protein form, and is activated by extracellular proteinase cleavage. MMP3 is capable of binding to zinc and calcium ions, and has metallo-endopeptidase activity. The active enzyme degrades collagen, gelatin, fibronectin, and laminin. It is involved in normal cellular processes such as wound repair and tissue remodeling. Mutations in the gene can result in coronary heart disease 6.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
基因别名 : CHDS6, MMP-3, MMP3, SL-1, STMY, STMY1, STR1
基因ID : (Human) 4314
基因符号 : MMP3
蛋白别名 : matrix metalloproteinase 3 (stromelysin 1, progelatinase), Matrix metalloproteinase-3, MMP-3, proteoglycanase, SL-1, Transin-1, EC 3.4.24.17, matrix metallo protease, MMP, MMPs, Stromelysin1, Transin1, Stromelysin-1
UniProt ID (Human) P08254
