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Invitrogen
Human/Mouse TGF beta-1 Uncoated ELISA Kit
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产品规格
检测灵敏度
8 pg/mL
测定范围
8-1,000 pg/mL
检测样本类型/体积
{Plasma=20 µL, Serum=20 µL, Supernatant=100 µL}
手工操作时间
1 hr 45 min
总时间
24 hr 30 min
均相（无需洗涤）
No
检测仪器
Absorbance Microplate Reader
产品规格
10 x 96 Tests
产品成分
Capture Antibody: Pre-titrated, purified antibody
Detection Antibody: Pre-titrated, biotin-conjugated antibody
Standard: Recombinant cytokine for generating standard curve and calibrating samples
Coating Buffer: 10X PBS ELISA Coating Buffer
Assay Diluent: 5X Concentrated
Detection Enzyme: Pre-titrated Avidin-HRP
Substrate Solution: Tetramethylbenzidine (TMB) Solution
Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards
96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
Detection Antibody: Pre-titrated, biotin-conjugated antibody
Standard: Recombinant cytokine for generating standard curve and calibrating samples
Coating Buffer: 10X PBS ELISA Coating Buffer
Assay Diluent: 5X Concentrated
Detection Enzyme: Pre-titrated Avidin-HRP
Substrate Solution: Tetramethylbenzidine (TMB) Solution
Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards
96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
运输条件
Wet ice
储存
2-8°C
蛋白名称
TGF beta-1
均相（无需洗涤）
Human, Mouse
检测试剂盒格式
Uncoated ELISA Kit
检测抗体结合物
Biotin
标签或染料
HRP
关于本试剂盒
The Human/Mouse Transforming Growth Factor beta 1 (TGFβ1) Uncoated ELISA Kit contains pre-matched antibody pairs, and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human/mouse TGFβ1. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately.
原理方法
ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
靶标信息
TGF beta-1 (TGFB1, Transforming Growth Factor Beta 1) is a polypeptide member of the transforming growth factor beta superfamily of cytokines, found almost ubiquitously in tissues. Transforming growth factor (TGF)-b is stored in the extracellular matrix as a latent complex with its pro-domain. Activation of TGF beta-1 requires the binding of aV integrin to an RGD sequence in the prodomain and exertion of force on this domain, which is held in the extracellular matrix by latent TGF-b binding proteins. Latent forms are complexes of TGF beta-1, an amino-terminal portion of the TGF-beta precursor, designated TGF-LAP (TGF-latency associated peptide), and a specific binding protein, known as LTBP. TGF beta-1 helps regulates proliferation, differentiation, adhesion, migration in many cell types. Many cells have TGF beta receptors, and the protein positively and negatively regulates many other growth factors. TGF beta-1 is cleaved into a latency-associated peptide and a mature TGF beta-1 peptide, and is found in either a latent form composed of a TGFB1 homodimer, a LAP homodimer, and a latent TGFB1-binding protein, or in an active form composed of a TGF beta-1 homodimer. The mature peptide may also form heterodimers with other TGF beta family members. The gene for TGF beta-1 is frequently upregulated in tumor cells, and mutations in this gene result in Camurati-Engelmann disease and cystic fibrosis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
基因别名 : CED, DPD1, LAP, TGF-beta1, TGFB, Tgfb-1, TGFB1, TGFbeta, TGFbeta1
基因ID : (Human) 7040, (Mouse) 21803
基因符号 : TGFB1, Tgfb1
蛋白别名 : latency-associated peptide, prepro-transforming growth factor beta-1, regulatory protein, TGF-beta 1, TGF-beta-1, LAP, TGF b, TGF b 1, transforming growth factor b1, transforming growth factor beta, transforming growth factor β1, TGF β 1, TGF β1, TGF-B1, TGF-beta 1 protein, TGFβ1, Transforming growth factor, TGF beta, tgf beta 1, tgf beta1, TGF beta-1, TGF beta-1 protein, TGF β, transforming growth factor beta-1, Transforming growth factor beta-1 proprotein, transforming growth factor-beta 1
UniProt ID (Human) P01137, (Mouse) P04202
