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Invitrogen
Human Neuropilin 1 Rapid ELISA Kit
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产品规格
检测灵敏度
0.47 ng/mL
测定范围
0.78-50 ng/mL
检测样本类型/体积
{Plasma=50 µL, Serum=50 µL}
手工操作时间
15 min
总时间
1 hr 30 min
均相（无需洗涤）
No
批间差异
<10%
批内差异
<10%
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate, Standard, HRP Conjugate, Standard & Sample Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
运输条件
Wet ice
储存
Refer to product documentation for component specific storage temperature.
蛋白名称
Neuropilin 1 (CD304)
均相（无需洗涤）
Human
检测试剂盒格式
Rapid ELISA Kit
检测抗体结合物
HRP
标签或染料
Unconjugated
关于本试剂盒
The Human NRP1 (Neuropilin 1) ELISA quantitates NRP1 in serum and plasma.
原理方法
The Human Neuropilin 1 (NRP1) solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the concentration of Neuropilin 1 (NRP1) in biological samples. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are added into these wells and bind to the immobilized (capture) antibody.
The sandwich complex is formed by the addition of a second (HRP-linked) detection antibody specific to Neuropilin 1 (NRP1). Excess reagents are washed from the plate.
A substrate solution is added that reacts with the enzyme-antibody-target complex to produce a measurable signal. The enzyme-substrate reaction is terminated by the addition of stop solution. The intensity of the signal, measured spectrophotometrically at 450 ± 2 nm, is directly proportional to the concentration of Neuropilin 1 (NRP1) present in the original specimen.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
靶标信息
Neuropilin 1 is a 923 amino acid containing membrane bound coreceptor to a tyrosine kinase receptor belonging to the neuropilin family with two CUB domains, two F5/8 type C domains and a MAM domain. Neuropilin 1 forms a heterodimer with NRP2 (Neuropilin 2) and is known to bind to plexin (PLXNB1) to form semaphorin receptors. Expressed mostly in the nervous system this receptor is implicated in a wide range of functions including in the development of the cardiovascular system, angiogenesis, repulsive axon guidance, formation of certain neuronal circuits, cell survival, control of neuronal migration and organogenesis outside the nervous system. This semaphorin receptor functions as receptors for both the semaphorins and vascular endothelial growth factor and is also a regulator of VEGF-induced angiogenesis. Neuropilin 1 may be involved in aggressive advanced prostate carcinoma and in the progression of breast cancer.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
基因别名 : BDCA4, CD304, NP1, NRP, NRP1, VEGF165R
基因ID : (Human) 8829
基因符号 : NRP1
蛋白别名 : alternatively spliced, lacks exon 16, CD304, Neuropilin, Neuropilin1, NRP 1, sNRP 1, sNRP1, soluble NRP 1, soluble NRP1, Neuropilin-1, receptor for VEGF165 and semaphorins class 3, transmembrane receptor, unnamed protein product, Vascular endothelial cell growth factor 165 receptor, VEGF 165 receptor
UniProt ID (Human) O14786
