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Invitrogen
Human Proprotein Convertase 9/PCSK9 Rapid ELISA Kit
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产品规格
检测灵敏度
0.94 ng/mL
测定范围
1.56-100 ng/mL
检测样本类型/体积
{Plasma=50 µL, Serum=50 µL}
手工操作时间
15 min
总时间
1 hr 30 min
均相（无需洗涤）
No
批间差异
<10%
批内差异
<10%
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate, Standard, HRP Conjugate, Standard & Sample Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
运输条件
Wet ice
储存
Refer to product documentation for component specific storage temperature.
蛋白名称
PCSK9
均相（无需洗涤）
Human
检测试剂盒格式
Rapid ELISA Kit
检测抗体结合物
HRP
标签或染料
Unconjugated
关于本试剂盒
The Human PCSK9 (Proprotein Convertase Subtilisin/Kexin Type 9) ELISA quantitates PCSK9 in serum and plasma.
原理方法
The Human Proprotein Convertase Subtilisin/Kexin Type 9 (PCSK9) solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the concentration of Proprotein Convertase Subtilisin/Kexin Type 9 (PCSK9) in biological samples. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are added into these wells and bind to the immobilized (capture) antibody.
The sandwich complex is formed by the addition of a second (HRP-linked) detection antibody specific to Proprotein Convertase Subtilisin/Kexin Type 9 (PCSK9). Excess reagents are washed from the plate.
A substrate solution is added that reacts with the enzyme-antibody-target complex to produce a measurable signal. The enzyme-substrate reaction is terminated by the addition of stop solution. The intensity of the signal, measured spectrophotometrically at 450 ± 2 nm, is directly proportional to the concentration of Proprotein Convertase Subtilisin/Kexin Type 9 (PCSK9) present in the original specimen.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
靶标信息
PCSK9 is a proprotein convertase belonging to the proteinase K subfamily of the secretory subtilase family. This protein is synthesized as a soluble zymogen that undergoes autocatalytic intramolecular processing in the endoplasmic reticulum. The protein may function as a proprotein convertase. The protein plays a role in cholesterol homeostasis and may have a role in the differentiation of cortical neurons.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
基因别名 : FH3, FHCL3, HCHOLA3, LDLCQ1, NARC-1, NARC1, PC9, PCSK9, PSEC0052
基因ID : (Human) 255738
基因符号 : PCSK9
蛋白别名 : convertase subtilisin/kexin type 9 preproprotein, hypercholesterolemia, OTTHUMP00000009833, NARC-1, neural apoptosis regulated convertase 1, Neural apoptosis-regulated convertase 1, PC9, Proprotein convertase 9, Proprotein convertase subtilisin/kexin type 9, Subtilisin/kexin-like protease PC9, unnamed protein product
UniProt ID (Human) Q8NBP7
