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Invitrogen
Human RNASE3 Rapid ELISA Kit
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产品规格
检测灵敏度
23.44 pg/mL
测定范围
39.06-2500 pg/mL
检测样本类型/体积
{Plasma=50 µL, Serum=50 µL}
手工操作时间
15 min
总时间
1 hr 30 min
均相（无需洗涤）
No
批间差异
<10%
批内差异
<10%
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate, Standard, HRP Conjugate, Standard & Sample Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
运输条件
Wet ice
储存
Refer to product documentation for component specific storage temperature.
蛋白名称
RNASE3
均相（无需洗涤）
Human
检测试剂盒格式
Rapid ELISA Kit
检测抗体结合物
HRP
标签或染料
Unconjugated
关于本试剂盒
The Human RNASE3 (Ribonuclease A3/Eosinophil Cationic Protein) ELISA quantitates RNASE3 in serum and plasma.
原理方法
The Human Ribonuclease A3/Eosinophil Cationic Protein (RNASE3) solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the concentration of Ribonuclease A3/Eosinophil Cationic Protein (RNASE3) in biological samples. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are added into these wells and bind to the immobilized (capture) antibody.
The sandwich complex is formed by the addition of a second (HRP-linked) detection antibody specific to Ribonuclease A3/Eosinophil Cationic Protein (RNASE3). Excess reagents are washed from the plate.
A substrate solution is added that reacts with the enzyme-antibody-target complex to produce a measurable signal. The enzyme-substrate reaction is terminated by the addition of stop solution. The intensity of the signal, measured spectrophotometrically at 450 ± 2 nm, is directly proportional to the concentration of Ribonuclease A3/Eosinophil Cationic Protein (RNASE3) present in the original specimen.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
靶标信息
ECP (eosinophil cationic protein), also known as RNS3 or RNASE3 (ribonuclease, RNase A family, 3), is a 160 amino acid potent cytotoxic secretory protein that belongs to the pancreatic ribonuclease family. ECP localizes to the granule matrix of the eosinophil and is involved in the immune response system. ECP is a cytotoxin, neurotoxin and helminthotoxin that is secreted by activated human eosinophils. ECP exhibits anti-parasitic, antibacterial and ribonucleolytic activities. It has been suggested that ECP induces the neurotoxic effect known as the Gordon phenomenon, a syndrome manifested by ataxia, muscular rigidity, paralysis and tremor that may lead to death. ECP is considered a marker of eosinophilic inflammation and high levels have been found in cases of active asthma and other allergic diseases.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
基因别名 : ECP, RAF1, RNASE3, RNS3
基因ID : (Human) 6037
基因符号 : RNASE3
蛋白别名 : cytotoxic ribonuclease, ECP, Eosinophil cationic protein, Ribonuclease 3, ribonuclease, RNase A family, 3, RNase 3, unnamed protein product
UniProt ID (Human) P12724
