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Invitrogen
Human VWF Rapid ELISA Kit
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Product Specifications
Analytical sensitivity
0.23 ng/mL
Assay range
0.39-25 ng/mL
Sample type/volume
Serum
50 µL
Plasma
50 µL
Hands-on time
15 min
Time-to-result
1 hr 30 min
Homogenous (no wash)
No
Interassay CV
<10%
Intraassay CV
<10%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate, Standard, HRP Conjugate, Standard & Sample Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
Shipping conditions
Wet ice
Storage
Refer to product documentation for component specific storage temperature.
Protein name
VWF
Species (tested)
Human
Assay kit format
Rapid ELISA Kit
Detector antibody conjugate
HRP
Label or dye
Unconjugated
About This Kit
The Human VWF (Von Willebrand Factor) ELISA quantitates VWF in serum and plasma.
Principle of the method
The Human Von Willebrand Factor (VWF) solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the concentration of Von Willebrand Factor (VWF) in biological samples. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are added into these wells and bind to the immobilized (capture) antibody.
The sandwich complex is formed by the addition of a second (HRP-linked) detection antibody specific to Von Willebrand Factor (VWF). Excess reagents are washed from the plate.
A substrate solution is added that reacts with the enzyme-antibody-target complex to produce a measurable signal. The enzyme-substrate reaction is terminated by the addition of stop solution. The intensity of the signal, measured spectrophotometrically at 450 ± 2 nm, is directly proportional to the concentration of Von Willebrand Factor (VWF) present in the original specimen.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Target information
Von Willebrand factor (vWf) is a series of self-aggregated structures, all derived from common glycoprotein subunits, synthesized in endothelial cells and megakaryocytes. The molecular mass of the monomeric vWf unit is 225 kDa. vWf binds to circulating factor VIIIc (antihemophilic factor), hereby stabilizing the latter thus being an important factor in the normal primary haemostasis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : F8VWF, VWD, VWF
Gene ID : (Human) 7450
Gene symbol : VWF
Protein Aliases : coagulation factor VIII VWF, Factor VIII related antigen, factor viii related antigen, von Willebrand Disease (vWD), Coagulation Factor VIII, unnamed protein product, von Willebrand antigen II precursor, von Willebrand factor, vWF
UniProt ID (Human) P04275
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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