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Invitrogen
Human hCG Rapid ELISA Kit
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产品规格
检测灵敏度
4.69 mIU/mL
测定范围
7.81-500 mIU/mL
检测样本类型/体积
{Plasma=50 µL, Serum=50 µL}
手工操作时间
15 min
总时间
1 hr 30 min
均相（无需洗涤）
No
批间差异
<10%
批内差异
<10%
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate, Standard, HRP Conjugate, Standard & Sample Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
运输条件
Wet ice
储存
Refer to product documentation for component specific storage temperature.
蛋白名称
hCG
均相（无需洗涤）
Human
检测试剂盒格式
Rapid ELISA Kit
检测抗体结合物
HRP
标签或染料
Unconjugated
关于本试剂盒
The Human HCG (Chorionic Gonadotropin) ELISA quantitates HCG in serum and plasma.
原理方法
The Human Chorionic Gonadotropin (HCG) solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the concentration of Chorionic Gonadotropin (HCG) in biological samples. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are added into these wells and bind to the immobilized (capture) antibody.
The sandwich complex is formed by the addition of a second (HRP-linked) detection antibody specific to Chorionic Gonadotropin (HCG). Excess reagents are washed from the plate.
A substrate solution is added that reacts with the enzyme-antibody-target complex to produce a measurable signal. The enzyme-substrate reaction is terminated by the addition of stop solution. The intensity of the signal, measured spectrophotometrically at 450 ± 2 nm, is directly proportional to the concentration of Chorionic Gonadotropin (HCG) present in the original specimen.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
靶标信息
Human chorionic gonadotropin (hCG) is a placental hormone that stimulates secretion of the pregnancy-sustaining steroid progesterone. hCG is a member of a family of glycoprotein hormones including pituitary hormones lutropin (luteinizing hormone;LH), follitropin (follicle-stimulating hormone; FSH) and thyrotropin (thyroid-stimulating hormone; TSH). Members of this family are all composed of disulfide-rich heterodimers with two dissimilar subunits namely alpha- and beta-subunits. The alpha subunit is common to all the members of the family within a given species. The beta subunits are distinct in each of the horomones and confer the receptor and biological specificity.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
基因别名 : CG-ALPHA, CGA, CGB, CGB3, CGB5, CGB7, CGB8, FSHA, GPA1, GPHa, GPHA1, HCG, hCGB, LHA, LHB, TSHA
基因ID : (Human) 1081, (Human) 1082, (Human) 93659, (Human) 94115
基因符号 : CGA, CGB3, CGB5, CGB8
蛋白别名 : Anterior pituitary glycoprotein hormones common subunit alpha, CG-alpha, CG-beta, CGA, Choriogonadotropin alpha chain, Choriogonadotropin subunit beta, Choriogonadotropin subunit beta 3, chorionic gonadotrophin chain beta, Chorionic gonadotrophin subunit alpha, chorionic gonadotropin beta 3 subunit, chorionic gonadotropin beta 5 subunit, chorionic gonadotropin beta 8 subunit, chorionic gonadotropin beta chain, chorionic gonadotropin beta subunit 3, chorionic gonadotropin beta subunit 5, chorionic gonadotropin beta subunit 8, Chorionic gonadotropin chain beta, chorionic gonadotropin human, human chorionic gonadotropin, chorionic gonadotropin, alpha polypeptide, beta polypeptide, Follicle-stimulating hormone alpha chain, follicle-stimulating hormone alpha subunit, Follitropin alpha chain, FSH-alpha, Glycoprotein hormones alpha chain, LSH-alpha, Luteinizing hormone alpha chain, luteinizing hormone beta subunit, Lutropin alpha chain, Thyroid-stimulating hormone alpha chain, Thyrotropin alpha chain, TSH-alpha, unnamed protein product
UniProt ID (Human) P01215, (Human) P0DN86, (Human) P0DN86, (Human) P0DN86
