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Invitrogen
Monkey ICAM-1 (Soluble) Matched Antibody Pair
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产品规格
检测灵敏度
6.3 U/mL
测定范围
6.3-100 U/mL
检测样本类型/体积
{Plasma=10 µL, Serum=10 µL, Supernatant=10 µL}
手工操作时间
24 hr
总时间
1 hr 10 min
均相（无需洗涤）
No
检测仪器
Absorbance Microplate Reader
产品规格
10 x 96 Tests
产品成分
Capture (coating) Antibody
Detection Antibody
Standard
Sample Diluent
Detection Antibody
Standard
Sample Diluent
运输条件
Wet ice
储存
-20°C
蛋白名称
ICAM-1 (CD54)
Target site-specificity
Target sub-specificity
Soluble
检测试剂盒格式
Matched Antibody Pair
检测抗体结合物
HRP
标签或染料
HRP
关于本试剂盒
The Monkey ICAM-1 Matched Antibody Pair Kit comes with pre-matched antibody pairs, standards, and sample diluent to develop your own enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of moneky ICAM-1. Buffer reagents and microplates needed to complete the ELISA reaction are sold separately.
原理方法
ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
靶标信息
ICAM-1 (CD54) is an 85-110 kDa single-chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. ICAM-1 has 7 potential N-linked glycosylation sites and shares considerable amino acid sequence homology with ICAM-3 (CD50) and ICAM-2 (CD102). ICAM-1 binds to integrins of type CD11a/CD18 (leukocyte adhesion molecule, LFA-1), or CD11b/CD18 (Mac-1) and is exploited by Rhinovirus as a receptor. ICAM-1 is expressed by activated endothelial cells and detected on epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils, with lower levels that increase upon inflammation. ICAM-1 is also detected in some carcinoma and melanoma cells. Soluble ICAM-1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名 : ICAM-1
