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Invitrogen
Mouse Basic IL-12 ELISA Kit
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产品规格
检测灵敏度
4 pg/mL
测定范围
31.3-2,000 pg/mL
检测样本类型/体积
{Serum=50 µL, Supernatant=50 µL}
总时间
3 hr 10 min
均相（无需洗涤）
No
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Coated Microwell Strips
Biotin-Conjugate
Streptavidin-HRP
Standard
Assay Buffer Concentrate
Wash Buffer Concentrate
Substrate Solution
Stop Solution
Adhesive Film
Biotin-Conjugate
Streptavidin-HRP
Standard
Assay Buffer Concentrate
Wash Buffer Concentrate
Substrate Solution
Stop Solution
Adhesive Film
运输条件
Wet ice
储存
2-8°C
蛋白名称
IL-12 p70
蛋白家族
Hormones & Receptors
均相（无需洗涤）
Mouse
Species (reported)
Mouse
检测试剂盒格式
Basic ELISA Kit
检测抗体结合物
HRP
标签或染料
HRP
关于本试剂盒
The Mouse Interleukin-12 (Ms IL-12) ELISA kit enables the precise quantification of in mouse serum or cell culture medium. This highly specific assay is designed to exclusively detect both endogenous and recombinant forms of Ms IL-12.
原理方法
The Mouse IL-12 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) employs a meticulously paired set of antibodies to capture and measure the target analyte. The wells of the microplate are pre-coated with a target-specific antibody, facilitating the binding of the analyte during the assay. Following the addition of samples, standards to the wells, they bind to the immobilized (capture) antibody. The formation of a sandwich complex is achieved by introducing a second (detector) antibody. Subsequently, a substrate solution is added, initiating a reaction with the enzyme-antibody-target complex and generating a measurable signal. The intensity of this signal is directly proportional to the concentration of the target present in the original specimen.
靶标信息
IL-12 p70, also known as interleukin-12, is a cytokine that plays a critical role in the immune system's response to infections and cancer. It is produced by immune cells, including dendritic cells, macrophages, and B cells, in response to certain pathogens and tumor cells. IL-12 p70 stimulates the production of interferon-gamma and other cytokines, which promote the activation and proliferation of T cells and natural killer cells. IL-12 p70 has been studied as a potential therapeutic target for various diseases and conditions, including infectious diseases, autoimmune disorders, and cancer. It has been shown to enhance immune responses against certain infections and tumors, and to modulate inflammatory responses in autoimmune diseases.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
基因别名 : Il-12a, Il-12b, IL-12p35, Il-12p40, Il12a, Il12b, Il12p40, Ll12a, p35, p40
基因ID : (Mouse) 16159, (Mouse) 16160
基因符号 : Il12a, Il12b
蛋白别名 : CLMF p35, CLMF p40, Cytotoxic lymphocyte maturation factor 35 kDa subunit, Cytotoxic lymphocyte maturation factor 40 kDa subunit, IL-12 subunit p35, IL-12 subunit p40, IL-12A, IL-12B, 20C2, il 12, IL 12p70, IL-12 p75, IL12A/B, IL12AB, ILN, Interleukin, Interleukin-12 p70, MGC151228, MGC151232, Interleukin-12 subunit alpha, Interleukin-12 subunit beta
UniProt ID (Mouse) P43431, (Mouse) P43432
