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Invitrogen
Mouse CCL6 Rapid ELISA Kit
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产品规格
检测灵敏度
4.69 pg/mL
测定范围
7.81-500 pg/mL
检测样本类型/体积
{Plasma=50 µL, Serum=50 µL}
手工操作时间
15 min
总时间
1 hr 30 min
均相（无需洗涤）
No
批间差异
<10%
批内差异
<10%
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate, Standard, HRP Conjugate, Standard & Sample Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
运输条件
Wet ice
储存
Refer to product documentation for component specific storage temperature.
蛋白名称
CCL6
均相（无需洗涤）
Mouse
检测试剂盒格式
Rapid ELISA Kit
检测抗体结合物
HRP
标签或染料
Unconjugated
关于本试剂盒
The Mouse CCL6 (Chemokine C-C-Motif Ligand 6) ELISA quantitates CCL6 in serum, plasma and other biological fluids.
原理方法
The Mouse Chemokine C-C-Motif Ligand 6 (CCL6) solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the concentration of Chemokine C-C-Motif Ligand 6 (CCL6) in biological samples. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are added into these wells and bind to the immobilized (capture) antibody.
The sandwich complex is formed by the addition of a second (HRP-linked) detection antibody specific to Chemokine C-C-Motif Ligand 6 (CCL6). Excess reagents are washed from the plate.
A substrate solution is added that reacts with the enzyme-antibody-target complex to produce a measurable signal. The enzyme-substrate reaction is terminated by the addition of stop solution. The intensity of the signal, measured spectrophotometrically at 450 ± 2 nm, is directly proportional to the concentration of Chemokine C-C-Motif Ligand 6 (CCL6) present in the original specimen.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
靶标信息
Chemokine(C-C motif) ligand 6(CCL6) is a small cytokine belonging to the CC chemokine family that has only been identified in rodents. In mice, CCL6 is expressed in cells from neutrophil and macrophage lineages, and can be greatly induced under conditions suitable for myeloid cell differentiation. It is highly expressed in bone marrow cultures that have been stimulated with the cytokine GM-CSF. Some low levels of gene expression also occur in certain cell lines of myeloid origin(e.g. the immature myeloid cell lines DA3 and 32D cl3, and the macrophage cell line P388D) that can also be greatly induced in culture with GM-CSF. However, in activated T cell lines, expression of CCL6 is greatly reduced. CCL6 can also be induced in the mouse lung by the cytokine interleukin 13. Mouse CCL6 is located on chromosome 11. The cell surface receptor for CCL6 is believed to be the chemokine receptor CCR1.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
基因别名 : C10, Ccl6, MRP-1, Scya6
基因ID : (Mouse) 20305
基因符号 : Ccl6
蛋白别名 : C-C motif chemokine 6, CC chemokine C10, Protein C10, small inducible cytokine A6, Small-inducible cytokine A6
UniProt ID (Mouse) P27784
