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Invitrogen
Multispecies GSK3 beta (Total/Phospho) InstantOne™ ELISA Kit
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Multispecies GSK3 beta (Total/Phospho) InstantOne™ ELISA Kit
Representative data for GSK3 beta (Total/Phospho) Multispecies InstantOne™ ELISA. MCF7 cells were treated with either the PI3 Kinase inhibitor Wortmannin (10 µM) (-) or insulin (10 ng/mL for 10 min.) (+). Cell lysates were prepared as described in the InstantOne ELISA User Manual. Using the GSK3 beta (Total/Phospho) InstantOne ELISA, the cell lysates were analyzed for levels of Total GSK3 beta (left) and... View More
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产品规格
检测样本类型/体积
{Cell Lysate=20 µL}
手工操作时间
1 hr 20 min
总时间
1 hr 10 min
均相（无需洗涤）
No
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Capture Antibody
Detection Antibody Reagent
InstantOne™ Assay Plate
Cell Lysis Buffer
Enhancer Solution
Wash Buffer (10X)
Substrate Reagent (TMB)
Stop Solution
Positive Control Cell Lysate (lyophilized)
Plate seal
Detection Antibody Reagent
InstantOne™ Assay Plate
Cell Lysis Buffer
Enhancer Solution
Wash Buffer (10X)
Substrate Reagent (TMB)
Stop Solution
Positive Control Cell Lysate (lyophilized)
Plate seal
运输条件
Wet ice
储存
2-8°C
蛋白名称
GSK3B
Target site-specificity
Target sub-specificity
Total
检测试剂盒格式
InstantOne ELISA
检测抗体结合物
Biotin
标签或染料
HRP
关于本试剂盒
The InstantOne™ ELISA is specifically engineered for accurate measurement of total and phosphorylated human and mouse GSK3b (Ser9) in cell lysates. Detection of total and phosphorylated rat GSK3b (Ser9) is also expected. The InstantOne™ ELISA kit allows for fast analysis of samples in approximately one hour. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate.
靶标信息
GSK3B is a proline-directed serine/threonine protein kinase involved in energy metabolism, neuronal cell development and body pattern formation. It is a negative regulator of glucose homeostasis and is involved in energy metabolism, inflammation, ER-stress, mitochondrial dysfunction, and apoptotic pathways. GSK3B is a critical component of the Wnt/beta-catenin signaling pathway, and its activity results in the inhibition of Wnt signaling by mediating the degradation of beta-catenin. GSK3B is also involved in the Hedgehog, Notch, and insulin signaling pathways.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名 : GSK-3 beta, Serine/threonine-protein kinase GSK3B
