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Invitrogen
Rat Transferrin Rapid ELISA Kit
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产品规格
检测灵敏度
0.38 ng/mL
测定范围
0.63-40 ng/mL
检测样本类型/体积
{Serum=50 µL, Plasma=50 µL}
手工操作时间
15 min
总时间
1 hr 30 min
均相（无需洗涤）
No
批间差异
<10%
批内差异
<10%
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate, Standard, HRP Conjugate, Standard & Sample Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
运输条件
Wet ice
储存
Refer to product documentation for component specific storage temperature.
蛋白名称
Transferrin
均相（无需洗涤）
Rat
检测试剂盒格式
Rapid ELISA Kit
检测抗体结合物
HRP
标签或染料
Unconjugated
关于本试剂盒
The Rat TF (Transferrin) ELISA quantitates TF in serum and plasma.
原理方法
The Rat Transferrin (TF) solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the concentration of Transferrin (TF) in biological samples. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are added into these wells and bind to the immobilized (capture) antibody.
The sandwich complex is formed by the addition of a second (HRP-linked) detection antibody specific to Transferrin (TF). Excess reagents are washed from the plate.
A substrate solution is added that reacts with the enzyme-antibody-target complex to produce a measurable signal. The enzyme-substrate reaction is terminated by the addition of stop solution. The intensity of the signal, measured spectrophotometrically at 450 ± 2 nm, is directly proportional to the concentration of Transferrin (TF) present in the original specimen.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
靶标信息
Transferrins (Tf) are an approximately 80kDa blood plasma glycoprotein synthesized by the liver. They are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Transferrin is the primary blood iron transport protein and under normal conditions; approximately one-third of total blood transferrin contains bound iron. Measurement of blood transferrin levels can be used as an indicator for blood iron-carrying capacity and abnormalities of iron metabolism such as anaemia, iron overload and haemochromatosis.Serum transferrin may also have a further role in stimulating cell proliferation.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
基因别名 : Tf, Tfn, Trf
基因ID : (Rat) 24825
基因符号 : Tf
蛋白别名 : Beta-1 metal-binding globulin, DKFZp781D0156, transferin, Liver regeneration-related protein LRRG03, Serotransferrin, Siderophilin, Transferrin
UniProt ID (Rat) P12346
