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Invitrogen
Retinol Binding Protein ELISA Kit
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产品规格
检测灵敏度
1.36 ng/mL
测定范围
7.8-1,000 ng/mL
检测样本类型/体积
{Serum=10 µL, Plasma=10 µL, Urine=100 µL, Whole blood=100 µL}
手工操作时间
45 min
总时间
1 hr 30 min
均相（无需洗涤）
No
批间差异
12%
批内差异
5%
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Anti-IgG coated 96 well plate
Standard
Assay Buffer Concentrate
Capture Antibody
HRP-conjugated Antigen
Wash Buffer Concentrate
TMB Substrate
Stop Solution
Plate Sealer
Standard
Assay Buffer Concentrate
Capture Antibody
HRP-conjugated Antigen
Wash Buffer Concentrate
TMB Substrate
Stop Solution
Plate Sealer
运输条件
Dry ice
储存
2-8°C
蛋白名称
RBP1
均相（无需洗涤）
Human
检测试剂盒格式
Competitive ELISA
检测抗体结合物
HRP
标签或染料
Unconjugated
关于本试剂盒
The Retinol Binding Protein Competitive ELISA quantitates retinol binding protein (RBP) in serum, plasma, urine, saliva, dried fecal extracts or cell culture medium.|原理方法: The Retinol Binding Protein Competitive ELISA research-use-only kit is designed to quantitatively measure RBP independent of species. An RBP standard is provided to generate a standard curve for the assay and all samples are read off a user-generated standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with anti-sheep IgG antibodies. Anti-sheep RBP antibodies and RBP-peroxidase conjugate is added to the wells. The RBP-peroxidase conjugate and any RBP in the sample will compete to bind to the anti-sheep RBP antibodies. After incubation, the plate is washed and substrate is added. The substrate reacts with the bound RBP-peroxidase conjugate. After a 30 min incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader at 450 nm. The intensity of the generated color corresponds inversely to the amount of RBP present in the sample.|Rigorous validation: Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
靶标信息
This gene encodes the carrier protein involved in the transport of retinol (vitamin A alcohol) from the liver storage site to peripheral tissue. Vitamin A is a fat-soluble vitamin necessary for growth, reproduction, differentiation of epithelial tissues, and vision. Multiple transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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生物信息学
基因别名 : CRABP-I, CRBP, CRBP1, CRBPI, hCRBP1, RBPC
基因ID : (Human) 5947
基因符号 : RBP1
蛋白别名 : C CRABP-I, RBP, retinol, retinol binding, Retinol Binding Protein, cellular retinol binding protein 1, cellular retinol-binding protein I, RBP1, retinol-binding protein, retinol-binding protein 1, cellular, unnamed protein product
UniProt ID (Human) P09455
