16th Multinational Congress on Microscopy (MCM 2022)

Speaker: Abhay Kotecha

Cryo-electro tomography (cryo-ET) allows protein structure determination both in vitro and in its native cellular context. Through sub-tomogram averaging (STA), the recurring structures in cryo-ET data can be resolved to sub nanometer resolutions. Recently, an increasing number of sub-5 Å maps achieved by STA demonstrates that the high-resolution information in cryo-ET data can be harvested for in situ structural biology. Here we will show that the high quality cryo-ET data acquired by Krios G4 equipped with Selectris energy filter and Falcon 4i detector enable us to resolve apoferritin at 1.9 Å with STA and yeast ribosome at 5 Å, with large subunit resolved to 3.5 Å from lamella by cryo-FIB milling. We will also show high throughput cryoFIB milling with our new PlasmaFIB system and data collection and assessment with Tomography 5 and Tomo live.

Speaker: Mauro Porcu

Join this presentation to discover how you can benefit from our latest S/TEM technology.  With the most sensitive EDX detector, featuring a 4.45 srad solid angle and a newly designed octagon that allows live HT Switch, the Thermo Scientific Spectra Ultra S/TEM enables chemical characterization of materials too beam-sensitive for conventional EDX analysis. The Spectra Ultra aberration-corrected S/TEM offers an industry leading level of characterization capabilities for materials science and semiconductor applications at the highest resolution on a wide variety of samples.

Key Learnings:

1) The possibilities offered by the improved EDX detector such as in situ dynamic EDX and EDX tomography
2) How the live HT switch can be beneficial in optimizing experiments
3) The latest advancements in electron microscopy for materials sciences