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On-demand: mRNA & Oligonucleotides Series: Decoding Complexity
Webinar
Jan 27, 2023
- Mar 31, 2023
In this series, we will feature curated, focused content on mRNA therapeutics development and/or oligonucleotide applications. You will hear from industry experts focusing on mRNA, lipid nanoparticles, and oligonucleotides. View agenda and speakers’ biography here.
Register now to get free access to the whole series and full resources.
Darwin Asa, Ph.D. | Market Development Manager, Thermo Fisher Scientific
Dr. Darwin Asa received his PhD in Biochemistry from the University of Illinois. He has held a variety of product development and marketing positions. Dr. Asa joined Thermo Fisher Scientific in 2021 and is currently the Global Market Development Manager for Nucleic Acid Therapeutics, responsible for many of the materials used in the development and manufacturing of oligonucleotide therapeutics and mRNA vaccines and therapeutics.
- Critical considerations on raw materials including quality, consistency, flexibility, regulatory support, scalability.
A novel approach for direct characterization of mRNA therapeutics and vaccines using an LCMS based workflow
Keeley Murphy, Ph.D. | Senior Product Applications Manager, Thermo Fisher Scientific
Dr. Keeley Murphy is the software product manager for BioPharma Finder, working to develop solutions and workflows for oligonucleotide analysis and mRNA mapping.
- Direct measurement of mRNA with comprehensive sequence coverage at >85%
- Universal solutions applicable to all types of mRNAs
- Fast analysis and reproducible result
Lipid Nanoparticle (LNP) Component Analysis using Charged Aerosol Detector
Sissi White, M.Sc. | Senior Product Applications Specialist, Thermo Fisher Scientific
Sissi White is a Sr. Application Scientist of application team within the Chromatography and Mass spectrometry division of Thermo Fisher Scientific. She is responsible for developing and validating chromatographic methods and solutions to meet biopharmaceutical customers’ needs. She has 15+ years analytical and project management experience with HPLC (Micro-scale UPLC, UPLC, UPC2, HDX, SFC, Prep LC/SFC and LC-MS (small molecule, amino acids, peptides, LNP and oligos) within the pharmaceutical industry and within instrumentation vendors.
- Sensitive and unbiased detection of all LNP components, including cholesterol
- Fast and robust methods for the characterization of the lipid components in LNP formulations
- Accurate and sensitive detection of impurities in lipid raw material
Characterization of Lipid nanoparticle (LNP) composition using UHPLC-MS
Reiko Kiyonami, Ph.D. | Senior Product Applications Specialist, Thermo Fisher Scientific
Dr. Reiko Kiyonami is the Senior product applications specialist at Thermo Fisher Scientific. She joined Thermo Fisher Scientific in 1990 and has extensive hands-on experiences for characterization and quantification of large biologics and small molecules. Her recent focus is to develop advanced analytical solutions for new therapeutic modalities using Orbitrap based mass spectrometry.
- Confident confirmation of lipid components by LC-HRAM MS
- Sensitive detection and identification of impurities at 0.001%
- Simultaneous lipid component quantification and metabolites characterization
Comprehensive LC-MS characterization of synthetic single guide RNAs in CRISPR: From bottom-up to top-down MS analysis
Molly Blevins, Ph.D. | Scientist (Technology), Small Molecule Analytical Chemistry, Genentech Research & Development
Dr. Molly Blevins completed her PhD in analytical chemistry at UT Austin under supervision of Professor Jennifer Brodbelt. There, she focused on developing novel methods in bioanalytical mass spectrometry towards analysis of lipids, proteins, and nucleic acids. Today, she works as a Scientist at Genentech Research & Development (gRED) in the Small Molecule Analytical Chemistry (SMAC) group and focuses on developing new methods and evaluating new technologies for challenging new drug modalities. Her analytical chemistry interests lie in oligonucleotide analysis, native mass spectrometry, novel MS/MS dissociation methods, and capillary electrophoresis.
- Complete (100%) sequence coverage of sgRNA using primer protection
- Strategies to achieve high sequence coverage of large oligonucleotides (~100 nt) via a direct sequencing approach
Streamlining workflow from characterization to monitoring of therapeutic oligonucleotides impurities using Orbitrap based LC-HRAM-MS platforms
Hao Yang, Ph.D. | Senior Product Applications Specialist, Thermo Fisher Scientific
Dr. Hao Yang is a senior application scientist in the Pharma and Biopharma Vertical Marketing group. He has been with Thermo Fisher Scientific for 3 years primarily focusing on developing analytical methodologies to support our Pharma and Biopharma customers. Currently, he is focusing on developing new methods to characterize and monitor oligonucleotides such as DNA primers and therapeutic oligos, as well as bioanalysis of therapeutic oligos.
- Complete characterization and identification of oligonucleotide impurities through the combination of low mass deviation of the measured monoisotopic mass and good correlation of the experimental MS/MS fragmentation against the predicted spectra
- Seamless method migration from research and development to quality control using Thermo Scientific™ Chromeleon™ CDS eWorkflow procedure for direct method transfer and execution of the workflow
Purification of mRNA with POROS Oligo (dt)25 Affinity Resin
Jenny England, Ph.D. | Manager, Research & Development, Thermo Fisher Scientific
Dr. Jenny England is a Manager in the Application group in Purification and Pharma Analytics at Thermo Fisher Scientific. Dr. England currently leads the application group for process development of the POROS resin products for antibody, mRNA, plasmid, and viral vector purification. Additionally, Dr. England evaluates new and emerging technologies that can be applied to solve unmet customer needs in the bioproduction workflow.
- Optimized for your specific molecule to maximize mRNA purification
- Scalable from small (μL or mL) to large (L) scale
Characterization of mRNA vaccine 5' and 3' end products using BioPharmaFinder 5.1
Robert Ross, Ph.D. | Senior Product Applications Specialist, Thermo Fisher Scientific
Dr. Robert Ross is an Application Specialist at Thermo Fisher in our Franklin, MA lab. He obtained his PhD from the University of Cincinnati where he studied characterization of RNAs by LC-MS.
- Accurate and sensitive characterization of capped and uncapped fragments
- Accurate characterization of poly(A) tail distribution with mass error <20 ppmt
Darwin Asa, Ph.D. | Market Development Manager, Thermo Fisher Scientific
Dr. Darwin Asa received his PhD in Biochemistry from the University of Illinois. He has held a variety of product development and marketing positions. Dr. Asa joined Thermo Fisher Scientific in 2021 and is currently the Global Market Development Manager for Nucleic Acid Therapeutics, responsible for many of the materials used in the development and manufacturing of oligonucleotide therapeutics and mRNA vaccines and therapeutics.
- Critical considerations on raw materials including quality, consistency, flexibility, regulatory support, scalability.
A novel approach for direct characterization of mRNA therapeutics and vaccines using an LCMS based workflow
Keeley Murphy, Ph.D. | Senior Product Applications Manager, Thermo Fisher Scientific
Dr. Keeley Murphy is the software product manager for BioPharma Finder, working to develop solutions and workflows for oligonucleotide analysis and mRNA mapping.
- Direct measurement of mRNA with comprehensive sequence coverage at >85%
- Universal solutions applicable to all types of mRNAs
- Fast analysis and reproducible result
Lipid Nanoparticle (LNP) Component Analysis using Charged Aerosol Detector
Sissi White, M.Sc. | Senior Product Applications Specialist, Thermo Fisher Scientific
Sissi White is a Sr. Application Scientist of application team within the Chromatography and Mass spectrometry division of Thermo Fisher Scientific. She is responsible for developing and validating chromatographic methods and solutions to meet biopharmaceutical customers’ needs. She has 15+ years analytical and project management experience with HPLC (Micro-scale UPLC, UPLC, UPC2, HDX, SFC, Prep LC/SFC and LC-MS (small molecule, amino acids, peptides, LNP and oligos) within the pharmaceutical industry and within instrumentation vendors.
- Sensitive and unbiased detection of all LNP components, including cholesterol
- Fast and robust methods for the characterization of the lipid components in LNP formulations
- Accurate and sensitive detection of impurities in lipid raw material
Characterization of Lipid nanoparticle (LNP) composition using UHPLC-MS
Reiko Kiyonami, Ph.D. | Senior Product Applications Specialist, Thermo Fisher Scientific
Dr. Reiko Kiyonami is the Senior product applications specialist at Thermo Fisher Scientific. She joined Thermo Fisher Scientific in 1990 and has extensive hands-on experiences for characterization and quantification of large biologics and small molecules. Her recent focus is to develop advanced analytical solutions for new therapeutic modalities using Orbitrap based mass spectrometry.
- Confident confirmation of lipid components by LC-HRAM MS
- Sensitive detection and identification of impurities at 0.001%
- Simultaneous lipid component quantification and metabolites characterization
Comprehensive LC-MS characterization of synthetic single guide RNAs in CRISPR: From bottom-up to top-down MS analysis
Molly Blevins, Ph.D. | Scientist (Technology), Small Molecule Analytical Chemistry, Genentech Research & Development
Dr. Molly Blevins completed her PhD in analytical chemistry at UT Austin under supervision of Professor Jennifer Brodbelt. There, she focused on developing novel methods in bioanalytical mass spectrometry towards analysis of lipids, proteins, and nucleic acids. Today, she works as a Scientist at Genentech Research & Development (gRED) in the Small Molecule Analytical Chemistry (SMAC) group and focuses on developing new methods and evaluating new technologies for challenging new drug modalities. Her analytical chemistry interests lie in oligonucleotide analysis, native mass spectrometry, novel MS/MS dissociation methods, and capillary electrophoresis.
- Complete (100%) sequence coverage of sgRNA using primer protection
- Strategies to achieve high sequence coverage of large oligonucleotides (~100 nt) via a direct sequencing approach
Streamlining workflow from characterization to monitoring of therapeutic oligonucleotides impurities using Orbitrap based LC-HRAM-MS platforms
Hao Yang, Ph.D. | Senior Product Applications Specialist, Thermo Fisher Scientific
Dr. Hao Yang is a senior application scientist in the Pharma and Biopharma Vertical Marketing group. He has been with Thermo Fisher Scientific for 3 years primarily focusing on developing analytical methodologies to support our Pharma and Biopharma customers. Currently, he is focusing on developing new methods to characterize and monitor oligonucleotides such as DNA primers and therapeutic oligos, as well as bioanalysis of therapeutic oligos.
- Complete characterization and identification of oligonucleotide impurities through the combination of low mass deviation of the measured monoisotopic mass and good correlation of the experimental MS/MS fragmentation against the predicted spectra
- Seamless method migration from research and development to quality control using Thermo Scientific™ Chromeleon™ CDS eWorkflow procedure for direct method transfer and execution of the workflow
Purification of mRNA with POROS Oligo (dt)25 Affinity Resin
Jenny England, Ph.D. | Manager, Research & Development, Thermo Fisher Scientific
Dr. Jenny England is a Manager in the Application group in Purification and Pharma Analytics at Thermo Fisher Scientific. Dr. England currently leads the application group for process development of the POROS resin products for antibody, mRNA, plasmid, and viral vector purification. Additionally, Dr. England evaluates new and emerging technologies that can be applied to solve unmet customer needs in the bioproduction workflow.
- Optimized for your specific molecule to maximize mRNA purification
- Scalable from small (μL or mL) to large (L) scale
Characterization of mRNA vaccine 5' and 3' end products using BioPharmaFinder 5.1
Robert Ross, Ph.D. | Senior Product Applications Specialist, Thermo Fisher Scientific
Dr. Robert Ross is an Application Specialist at Thermo Fisher in our Franklin, MA lab. He obtained his PhD from the University of Cincinnati where he studied characterization of RNAs by LC-MS.
- Accurate and sensitive characterization of capped and uncapped fragments
- Accurate characterization of poly(A) tail distribution with mass error <20 ppmt
Register for Thermo Fisher On-demand mRNA & Oligonucleotides Series: Decoding complexity Webinar now!
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