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Gibco GlycanTune Total Feeds

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Achieve targeted glycan profiles within fed-batch workflows

Controlling glycosylation in fed-batch Chinese hamster ovary (CHO) processes remains a persistent challenge for process developers, particularly when glycan profiles must be tuned without changing base medium or feed architecture. Variability in galactosylation can affect protein quality attributes and create risk during development, scale-up, and regulatory review. Gibco GlycanTune Total Feeds address this challenge by using feed transition timing as a controllable process lever. By replacing standard feeds at a defined point in the culture, teams can shift glycan distributions from predominantly G0F toward higher G1F and G2F content in a predictable manner.

Benefits of GlycanTune Total Feeds

GlycanTune Total Feeds support workflow-level control of glycosylation while preserving the performance and consistency of established fed-batch CHO processes.

Key benefits include:

  • Enable predictable, timing-based shifts in glycan profiles from higher G0F toward increased G1F and G2F content
  • Support improved protein quality attributes without compromising cell growth or overall productivity
  • Reduce culture dilution through flexible super-concentration, helping increase usable bioreactor working volume
  • Simplify feed preparation and handling with chemically defined pH-neutral formulations that require water for reconstitution
  • Maintain operational consistency across runs, scales, and sites by integrating into existing feeding schedules and control strategies

Performance in glycan modulation workflows

GlycanTune Total Feeds are intended to carry glycan control forward as processes mature, from early development studies through large-scale fed-batch manufacturing. Glycan modulation strategies defined at small scale can be directly translated to larger bioreactor volumes, as the feeds align with established scale-up practices. This allows teams to define glycan control strategies early and maintain continuity as programs progress toward late-stage development and production.

Bar chart describing how GlycanTune Total Feeds support glycan modulation strategies

Figure 1. The timing of transition from Gibco EfficientFeed+ C+ supplement (EFC+) to GlycanTune C+ Total Feed (GTC+) makes it possible to target specific glycosylation profiles. This transition modulates G0F from 75% down to 32% while increasing G1F (1 and 2) and increasing G2F.

Integrate with existing CHO feed strategies


Rather than requiring changes to core media or feeding architectures, GlycanTune Total Feeds fit into the fed-batch strategies teams already rely on. They can be introduced as a standalone feed or alongside Gibco EfficientFeed Supplements, or substituted for existing feeds at a selected point in culture to apply glycan control. This approach preserves familiar growth and productivity behaviors while adding a practical, timing-based mechanism for modulating glycosylation

Applications for GlycanTune Total Feeds

GlycanTune Total Feeds are applied in CHO-based fed-batch workflows where control of glycosylation is critical to protein quality, consistency, and downstream performance. Typical use scenarios include:

  • Programs targeting defined galactosylation profiles to support comparability across runs
  • Processes requiring adjustment of G0F, G1F, and G2F distributions to align with reference molecules or internal quality targets
  • Development workflows where glycan control must be introduced

By using feed transition timing as a controllable variable, teams can apply glycan modulation as a focused intervention within existing upstream processes.

Select the right formulation

Select GlycanTune A+, B+, or C+ Total Feeds based on the base medium and feed strategy already in use, as each formulation is aligned with the corresponding Gibco EfficientFeed A+, B+, or C+ platform. Matching the GlycanTune formulation to the existing feed system or applying it as a standalone feed supports compatibility and predictable nutrient delivery while enabling glycan modulation through controlled transition timing. The extent and direction of glycan shift can then be tuned by adjusting when the transition occurs during culture, allowing process developers to align glycan outcomes with protein quality targets.

 

Table 1. Compare Gibco GlycanTune Total Feed Formulations
 

GlycanTune formulation

Aligned feed platform

Typical base media compatibility

Primary use focus

Gibco GlycanTune A+ Total Feed

Gibco EfficientFeed A+ Supplement

Gibco CD OptiCHO Medium, Gibco CD CHO Medium

Early-stage development and moderate glycan shifts

Gibco GlycanTune B+ Total Feed

Gibco EfficientFeed B+ Supplement

CD CHO Medium, CD OptiCHO Medium

Balanced productivity and glycan modulation

Gibco GlycanTune C+ Total Feed

Gibco EfficientFeed C+ Supplement

Gibco Dynamis Medium, CD CHO Medium

High-density cultures and broader glycan adjustment

Ordering information

货号 产品名称 规格 Price 数量
A2971901 GlycanTune™ A+ Total Feed, 100 L Each
279,724.00
A2971904 GlycanTune™ A+ Total Feed, 1 L Each
4,180.00
A2971905 GlycanTune™ A+ Total Feed, 10 L Each
33,481.00

货号 产品名称 规格 Price 数量
A2972001 GlycanTune™ B+ Total Feed, 100 L Each
249,588.00
A2972004 GlycanTune™ B+ Total Feed, 1 L Each
4,180.00
A2972005 GlycanTune™ B+ Total Feed, 10 L Each
27,191.00

货号 产品名称 规格 Price 数量
A2972101 GlycanTune™ C+ Total Feed, 100 L Each
268,879.00
A2972104 GlycanTune™ C+ Total Feed, 1 L Each
4,242.00
A2972105 GlycanTune™ C+ Total Feed, 10 L Each
32,502.00

Discover other feeds and supplements

GlycanTune Total Feeds are part of the broader Gibco feeds and supplements portfolio designed to support integrated upstream bioprocessing workflows. Used alongside feeding and productivity solutions, they enable teams to address glycan control, nutrient delivery, and performance objectives within a single, cohesive fed-batch strategy.

Gibco EfficientFeed+ Supplements

Build fed-batch strategies around Gibco EfficientFeed+ Supplements to support robust cell growth and efficient nutrient delivery throughout culture. These chemically defined feeds help maximize bioreactor utilization and offer a stable foundation for productivity-focused and glycan-modulation workflows.

Gibco FunctionMAX TiterEnhancer

When productivity begins to decline late in culture, Gibco FunctionMAX TiterEnhancer can be introduced to reinforce specific productivity. Designed for high-cell-density fed-batch processes, it enhances existing feeding strategies without requiring changes to core media or schedules.

Explore mAb upstream solutions


Advance upstream performance by connecting feeding strategies to earlier development decisions that shape monoclonal antibody (mAb) process robustness. EfficientFeed+ Supplements fit within integrated CHO-based upstream workflows that combine cell line development, media and feed selection, and early bioreactor evaluation to address variability and support consistent scale-up.

Frequently asked questions

GlycanTune Total Feeds are introduced by transitioning from the standard feed at a defined point during fed-batch culture. An earlier transition supports a greater shift from G0F toward increased G1F and G2F, while a later transition maintains higher G0F levels. The optimal timing is determined through a simple, small-scale experiment that evaluates glycan outcomes alongside cell growth and productivity.

Feed transition timing acts as a controllable lever for glycan modulation in fed-batch CHO cultures. Introducing GlycanTune Total Feeds earlier in culture results in a larger shift from G0F toward increased G1F and G2F glycoforms, while later transitions preserve higher G0F levels. Experimental studies demonstrate a graded, predictable relationship between the transition point and the glycosylation profile, enabling targeted control without affecting growth or productivity.

GlycanTune Total Feeds are designed to integrate into established fed-batch CHO processes without requiring changes to base media or overall feeding architecture. They can temporarily replace standard feeds at a defined point in culture or be used alongside existing feed strategies to introduce glycan control. This flexibility allows teams to apply targeted glycan modulation while preserving familiar growth and productivity profiles.

Experimental studies show that glycan modulation using GlycanTune Total Feeds maintains cell growth and protein productivity when applied through controlled feed transitions. Shifting glycan profiles by adjusting transition timing does not negatively affect viable cell density or final titers compared with standard feeding approaches. This allows teams to improve glycan quality attributes while preserving the performance characteristics of established fed-batch CHO processes.

GlycanTune Total Feeds can be applied from early process development through scale-up and manufacturing-scale fed-batch operations. Small-scale studies are used to define transition timing and target glycan profiles, and the same strategy can be carried forward as processes move into larger bioreactors. This continuity supports consistent glycan control across development stages while maintaining established growth and productivity performance.

Cell culture resources


The Cell Culture Resource Hub showcases technical documents, application notes, and workflow guides to evaluate media, feed strategies, and culture conditions across development and scale-up. These resources offer practical guidance for improving consistency and understanding how different formulations perform in upstream processes.

Advance glycan control in your upstream workflow

For research use or further manufacturing. Not for diagnostic use or direct administration into humans or animals.