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Analytical methods like mid-infrared (MIR), near infrared (NIR), Fourier transform infrared (FTIR), and Raman spectroscopy, along with UV-Vis absorption spectroscopy provide rapid, accurate analysis capabilities and they are often complementary in nature. This compendium of application notes includes examples of the use of FT-NIR to predict protein concentrations in cell cultures, the application of FTIR to elucidate protein secondary structures, protein aggregation as identified through UV-Vis absorption spectroscopy, process analytical technology (PAT) that can be used as a real-time guide to downstream buffer control, and more.
Review these valuable techniques and the applications throughout the biopharmaceutical manufacturing workflow.
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