Targeted Metabolomics Workflow

The Stellar Mass Spectrometer expands experimental scale, permitting quantitative characterization of large number of metabolite targets in one experiment enabling high throughput needed to evaluate systems biology.

To gain a complete insight into their biological condition, metabolomics studies measure a wide range of endogenous metabolite levels. Targeted methods on Stellar MS allow for the quantification of extremely low femtomole concentrations, offering a linear dynamic range spanning up to five orders of magnitude with accurate coefficients of variation (CV).

For accurate biomarker measurement, high sensitivity and specificity is crucial to ensure that detected signals corresponds to the target analytes. MS3 acquisition effectively minimizes background noise and enhances specificity, especially for distinguishing isomeric compounds in complex samples. The Stellar Mass Spectrometer excels with its rapid tMS3 acquisition speeds of up to 40 Hz and automated method setup. This advanced tMS3 acquisition improves detection limits (LOD) and quantitation limits (LOQ), allowing for the simultaneous quantification of multiple analytes in a single spectrum, even with fast UHPLC gradients.

The Stellar Mass Spectrometer makes it possible to accelerate biomarker verification, while maintaining analyte capacity, sensitivity, selectivity, and specificity.

Accurate and reproducible metabolite quantitation

Experience significantly enhanced biomarker verification through advanced mass spectrometry capabilities and a robust targeted metabolomics workflow. With our targeted metabolomics workflow, you may:

• Accurately measure the concentrations of predefined metabolites within a sample
• Identify and validate biomarkers for disease diagnosis, prognosis, and therapeutic monitoring
• Investigate specific metabolic pathways and their alterations in different biological conditions
• Process a large number of samples efficiently, which is ideas for large-scale studies

SRM analysis on Thermo Scientific TSQ Triple Quadrupole Mass Spectrometers is the benchmark for targeted metabolomics. For targeted lists of known, well-characterized metabolites and compounds, TSQ Triple Quadrupole Mass Spectrometers offer sensitive, fast, and robust quantitation of metabolites.

For remarkable sensitivity and broad compound coverage, Stellar MS integrates the quantitative power of triple quadrupole technology with the rapid full-scan MSn acquisition capabilities of dual-pressure linear ion trap technology. Ideal for large-scale studies, Stellar MS enables high-throughput targeted quantitation workflow without compromising data quality. The proficiency in handling complex sample matrices ensures precise and efficient method development. Additionally, high scan speed and sensitivity facilitate deep and accurate quantitation, allowing for faster analysis and larger sample sizes. The seamless transition from discovery to validation phases accelerates the translation of metabolomic findings into actionable insights for precision medicine, diagnostics, and therapeutic interventions, positioning the Stellar MS as an indispensable tool in the field of metabolomics.

Alternatively, the AbsoluteIDQ^® p400 HR kit, the first standardized solution for broad targeted metabolic profiling developed and verified for use with Orbitrap mass spectrometers, covers key metabolites relevant to pathophysiological processes and disease specific markers. This workflow delivers quantification of hundreds of metabolites with high throughput and the reproducibility needed for rigorous and confident results.