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LFQ workflow
Our workflow is designed for maximum efficiency, using the Thermo Scientific AccelerOme Automated Sample Preparation Platform to boost throughput and minimize technical variability. Samples are then separated with precision using the Thermo Scientific Vanquish Neo UHPLC System paired with the Thermo Scientific μPAC Neo HPLC Column. Finally, Thermo Scientific mass spectrometers, powered by Orbitrap technology, deliver high-resolution accurate mass measurements for precise and reliable protein detection and quantification, even in complex samples.
What you get from our LFQ workflow
The need for analyzing large numbers of samples, especially in clinical and biomarker discovery studies or drug discovery and development screening, makes LFQ-based workflows an ideal choice for ensuring high throughput and accurate quantitative analyses regardless of the sample amount.
Accurate and precise quantitation
Experience accurate, precise and confident protein identification and quantitation with our Thermo Scientific LFQ workflow. Designed for complex proteomic samples, this workflow combines ultra-fast acquisition speeds with narrow-window DIA (nDIA) enabled by the Thermo Scientific Orbitrap Astral mass spectrometer series to deliver exceptional quantitative accuracy and precision. It consistently quantifies protein groups with coefficients of variation under 5% and achieves measured ratios within 10% of theoretical values, even in high-throughput settings. With reliable, reproducible results and uncompromised proteome depth, the Thermo Scientific LFQ workflow is the perfect solution for large-scale and clinically significant proteomics studies.
Quantitative accuracy of Thermo Scientific LFQ workflow using the Thermo Scientific Orbitrap Astral Zoom Mass Spectrometer equipped with a Thermo Scientific Vanquish Neo UHPLC System. Two mixtures, A and B, were prepared with different compositions of three proteomes. Mix A consisted of 65% HeLa digest, 30% yeast digest, and 5% E. coli digest. Mix B comprised of 65% HeLa digest, 15% yeast digest, and 20% E. coli digest. Both mixtures were analyzed on an Orbitrap Astral Zoom MS at sample loads ranging from 20 to 1000 ng. Box plots display the median log2 three-proteome ratio results derived from these sample compositions. The results show consistent accuracy of quantitation across the entire proteome of detected proteins versus known expected values.
Quantitative precision of Thermo Scientific LFQ workflow using the Orbitrap Astral Zoom MS. Peptide and protein group CVs of 20 ng (A) and 200 ng (B) HeLa measured at different throughputs. (C) Gain of total quantified, quantified <20%, and <10% CV peptides and protein groups at 300 SPD with the Orbitrap Astral Zoom MS. The results show consistency of quantitation at flexible throughput of analysis.
Quantitative accuracy and proteome depth of Thermo Scientific LFQ workflow using he Thermo Scientific Orbitrap Excedion Pro Hybrid Mass Spectrometer equipped with Vanquish Neo UHPLC system and Thermo Scientific FAIMS Pro Duo interface. A mixture was prepared with different compositions of three proteomes, which consisted of 65% HeLa digest, 30% yeast digest, and 5% E. coli digest. Samples were separated on a Thermo Scientific µPAC Neo UHPLC Column. Box plots display the median log2 three-proteome ratio results derived from these sample compositions. The results show consistent accuracy of quantitation across the entire proteome of detected proteins versus known expected values.
Identifications
The Thermo Scientific LFQ workflow makes it easy to uncover more proteins, and peptides and post-translational modifications (PTMs) in every experiment, even when working quickly or with limited sample amounts. Its advanced speed and sensitivity consistently deliver more identifications than ever before, ensuring no important details are missed. This powerful performance gives researchers greater confidence and deeper insights, even in demanding high-throughput studies.
Throughput
The Thermo Scientific LFQ workflow helps researchers analyze more samples in less time, making even large projects faster and easier to complete. Its advanced speed and efficiency let you keep up with demanding schedules without compromising the quality of your results. This high-throughput performance makes big studies and quick turnarounds simpler and more achievable.
Protein groups (A) and peptides (B) as identified by Orbitrap Astral Zoom MS equipped with a Vanquish Neo UHPLC system.
Sensitivity
Proteomics analysis by mass spectrometry often involves small amounts of material because biological samples, such as tissues or cell cultures, are typically available in limited quantities. The Thermo Scientific LFQ workflow is highly sensitive, enabling the detection and analysis of proteins at very low concentrations, thus reducing the need for large sample volumes. Advances in Orbitrap mass spectrometers have enhanced the efficiency and accuracy of proteomic analyses, allowing for meaningful results from minimal sample amounts, down to low picogram levels or even from single cells.
Identification of HeLa dilution series
Total protein groups identified in triplicate runs (n=3) of HeLa dilution series using a library-free search approach on an Orbitrap Astral Zoom mass spectrometer.
Robustness and consistency
To serve the need of biological investigations across large sample cohorts, a platform that offers robust, consistent performance for proteomics analysis is required. The Thermo Scientific LFQ workflow is highly robust, enabling the analysis of thousands of samples across months of continuous operation. Advances in Orbitrap mass spectrometers have enabled the achievement of robust, deep proteome profiling with consistency of performance over a much higher throughput than previously possible with MS-based proteomics, with some analytical workflows serving 300–500 samples per day.
Achieving consistent protein read-outs from the analysis of over 12,000 neat plasma samples during continuous operation over four months using the Orbitrap Astral MS equipped with the Vanquish Neo UHPLC system.
Comprehensive coverage of the proteome enabled by deep analysis with Orbitrap mass spectrometers
Discover the integrated Thermo Scientific LFQ workflow that delivers proven robustness, sensitivity, and confidence from sample to result. Our advanced platforms are engineered to meet the demands of large-scale proteomics studies, ensuring you get reproducible, high-quality data every time.
The Thermo Scientific LFQ workflow offers:
- Deep proteome coverage
- Simultaneous analysis of post-translational modifications
- Sensitivity for limited sample amount
- Quantitative accuracy and precision
- Automated sample preparation
- Identification of trends between samples and populations
- The ability to overcome the technical limitations associated with studying the most challenging samples
Identification and quantitation
Label-Free Quantitation workflow
Thermo Scientific LFQ workflow enabling broad, in-depth proteome coverage
What customers are saying about the Orbitrap Astral Zoom mass spectrometer
“The Orbitrap Astral MS has been a huge step forward in performance for us, but the data we have generated with the Orbitrap Astral Zoom MS is even more exciting – we can now routinely do 300 human proteomes per day. This allows us to do very systematic, systems biology type studies, where we get sufficient coverage of close to 8,000 proteins and more than 100,000 peptides across all the samples.”
Jesper Olsen, Ph.D.,
Deputy Center Director, Professor, Novo Nordisk Foundation Center for Protein Research, University of Copenhagen
For Research Use Only. Not for use in diagnostic procedures.