Antibody purification involves isolation of antibody from serum (polyclonal antibody), ascites fluid, or from the culture supernatant of a hybridoma cell line (monoclonal antibody). Protein A ligand is recommended specifically for polyclonal IgG from rabbit, pig, dog, or cat serum. It binds the heavy chain constant region (Fc) of IgG (CH2-CH3 region). Native Protein A is purified from Staphylococcus aureus (46.7 kDa; 4 IgG-binding sites). The POROS MabCapture A resins feature a recombinant Protein A (rPA) ligand produced in yeast that can be scaled up for bioprocess applications.

The MabCaptureC High Capacity Protein A is a high-performance chromatography resin for capturing mAbs and recombinant Fc-tagged fusion proteins. It provides high specific binding capacity and enhanced alkaline stability characteristics under clean-in-place conditions (CIP), for a more robust purification and resin regeneration.

We offer a wide selection of base supports and formats to purify antibodies using the Protein A ligand. Our portfolio is designed to meet small-scale (screening) up to large-scale (bioprocess) needs.

  • Multiple formats—loose resin, spin columns and kits, FPLC cartridges, and 96-well spin plates enable antibody purification from microgram to kilogram scales
  • Alkaline stability—engineered to withstand common CIP procedures with 0.2 M NaOH enabling efficient cleaning of the chromatography resin
  • High performance—resins are designed to maximize protein yield and reduce background
  • Economical—pricing is similar to or better than our leading competitors

Choose the right Protein A product for antibody purification

Product links in the last row of each column

 Pierce
Protein A Agarose		Pierce
Protein A Plus Agarose		Pierce
Recombinant Protein A Agarose		MabCaptureC High Capacity Protein A, Alkaline-Stable
Bead or resin size45–165 μm45–165 μm45–165 μm75 μm
Binding capacity12–19 mg human IgG/mL resin34 mg human IgG/mL resin15–17 mg human IgG/mL resin58 mg/ml at 5 min residence (80 mg/ml at 10 min residence) – 10% breakthrough
Maximum linear flow rate700 cm/hr700 cm/hr700 cm/hr300 cm/hr or less (150 cm/hr recommended)
SupportCrosslinked 6% beaded agaroseCrosslinked 6% beaded agaroseCrosslinked 6% beaded agaroseHighly cross-linked agarose
Recommended application scalebatch, pilotscreening, batchbatchscreening, batch, pilot, process
Formats availableloose beads, chromatography cartridgesloose beads, pre-packed spin columns and kits, 96-well spin platesloose beadsloose beads, packed spin columns, 96-well spin plates
Order product(s)20333
(loose resin)
88924
(1 mL cartridge)
89925
(5 mL cartridge)
44667
(spin kit)		22810
(loose resin)
45202
(spin plates)		20365A53031, 10 mL
A53033, 50 mL 
A53034, 200 mL 
1963662250, 250 mL 
196366201L, 1 L 
196366205L, 5 L
196366210L, 10 L 
A40004566, spin columns
A40004564, spin plates
 
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