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pSilencer™ 2.1-U6 neo
NOTE: This map applies only to our new and improved pSilencer 2.1-U6 neo vector which began shipping in Dec 2003 (Lots 113P06 & 113P07).
pSilencer™ 2.1-U6 neo Vector Map
This map and the annotated sequence below portrays the circular negative control vector provided with the kit. It includes the negative control siRNA template (66 base pairs) between the BamH I and Hind III sites.
pSilencer 2.1-U6 neo Annotated Promoter/Cloning Site Region
We recommend the following primers for sequencing:
M13F(-40): 5'-GTTTTCCCAGTCACGAC-3'
Rev: 5'-GAGTTAGCTCACTCATTAGGC-3'
pSilencer 2.1-U6 neo Restriction Sites
Enzymes with single Restriction Sites
| Site | Enzyme |
|---|---|
| 4456 | AatII |
| 1151 | AflIII |
| 2044 | AhdI |
| 1567 | AlwNI |
| 4008 | AvaI |
| 4029 | AvrII |
| 730 | BamHI |
| 3462 | BanII |
| 2549 | BcgI |
| 2583 | BcgI' |
| 3964 | BsaBI |
| 2105 | BsaI |
| 1026 | BsaXI |
| 996 | BsaXI` |
| 4039 | BseRI |
| 45 | BsmBI |
| 2903 | BsmI |
| 436 | DraIII |
| 3913 | EagI |
| 396 | EcoRI |
| 792 | HindIII |
| 3741 | MscI |
| 3139 | NruI |
| 1151 | PciI |
| 436 | PmlI |
| 2962 | PsiI |
| 3744 | PstI |
| 3304 | RsrII |
| 2524 | ScaI |
| 4261 | SexAI |
| 4010 | SmaI |
| 4034 | StuI |
| 3704 | Tth111I |
| 4008 | XmaI |
| 2643 | XmnI |
| 4454 | ZraI |
Non-Cutting Enzymes:
| Enzyme |
|---|
| AarI |
| Acc65I |
| AccI |
| AfeI |
| AflII |
| AgeI |
| AleI |
| AloI |
| AloI' |
| ApaI |
| AscI |
| AsiSI |
| BaeI |
| BaeI' |
| BbsI |
| BbvCI |
| BglII |
| BlpI |
| BmgBI |
| BmtI |
| Bpu10I |
| BsgI |
| BsiWI |
| BspEI |
| BsrGI |
| BstBI |
| BstEII |
| BstXI |
| BstZ17I |
| Bsu36I |
| BtrI |
| ClaI |
| EcoICRI |
| EcoNI |
| EcoRV |
| FseI |
| FspAI |
| HincII |
| HpaI |
| KpnI |
| MfeI |
| MluI |
| NheI |
| NotI |
| PacI |
| PflMI |
| PmeI |
| PpuMI |
| PshAI |
| PspOMI |
| PsrI |
| PsrI' |
| SacI |
| SacII |
| SalI |
| SanDI |
| SbfI |
| SfiI |
| SgfI |
| SgrAI |
| SnaBI |
| SpeI |
| SrfI |
| SwaI |
| TaqII' |
| XbaI |
| XcmI |
| XhoI |