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pSilencer™ adeno 1.0-CMV System: Shuttle Vector 1.0-CMV
We recommend the following primers for sequencing:
5' Sequencing Primer: 5'-ggatttccaagtctccac-3'
3' Sequencing Primer: 5'-agcaccttccagatctgc-3'
Detail of Multi-Cloning Site
Enzymes with single Restriction Sites
| Site | Enzyme |
|---|---|
| 2978 | AarI |
| 375 | Acc65I |
| 1321 | AflII |
| 158 | AflIII |
| 3310 | BbeI |
| 15 | BmtI |
| 1853 | BsaBI |
| 389 | BsaXI |
| 359 | BsaXI` |
| 5742 | BsrBI |
| 207 | BsrGI |
| 2974 | BstEII |
| 2398 | BstXI |
| 3554 | BstZ171 |
| 5696 | EarI |
| 5123 | FspI |
| 3306 | KasI |
| 379 | KpnI |
| 1712 | MfeI |
| 3307 | NarI |
| 620 | NdeI |
| 11 | NheI |
| 1085 | NotI |
| 3899 | NruI |
| 7 | NruI |
| 158 | PciI |
| 1959 | PflMI |
| 5271 | PvuI |
| 1092 | SalI |
| 5381 | ScaI |
| 3308 | SfoI |
| 202 | SgrAI |
| 726 | SnaBI |
| 1029 | SpeI |
| 3569 | StuI |
| 1549 | Tth111I |
| 970 | XhoI |
| 5500 | XmnI |
Non-Cutting Enzymes:
| Enzyme |
|---|
| AgeI |
| AleI |
| AloI |
| AloI' |
| AscI |
| AsiSI |
| AvrII |
| BaeI |
| BaeI' |
| BclI |
| BlpI |
| BsiWI |
| BspEI |
| BstBI |
| Bsu36I |
| ClaI |
| EcoNI |
| EcoRI |
| EcoRV |
| FalI |
| FalI' |
| FseI |
| FspAI |
| HindIII |
| HpaI |
| MluI |
| PmeI |
| PmlI |
| PsiI |
| PsrI |
| PsrI' |
| RsrII |
| SanDI |
| SapI |
| SbfI |
| SfiI |
| SgfI |
| SrfI |
| SwaI |
| TaqII' |
| XbaI |