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Our Specialty TaqMan Assay and Oligo Service offers solutions that extend beyond our catalog assay offerings, tailored to your real-time PCR needs. With this service you can further customize our TaqMan Assays—preformulated primers and probe sets for gene expression, SNP genotyping, copy number variation, and miRNA analysis—or design standalone TaqMan probes and primers for increased flexibility.
Our services provide a range of custom configurations to meet your specific experimental requirements, including alternative dye and quencher combinations, custom formulations, concentrations, and volumes, additional or modified primers and probes (including locked nucleic acid (LNA) probes), and specialized base modifications.
To get started, complete the request form below, and a member of our Specialty Orders Team will contact you.
For gene expression and SNP genotyping multiplexing support, visit our Assay Design Hub. For additional support, email Specialty_Oligos@thermofisher.com.
Our Specialty TaqMan Assay and Oligo Service supports the following custom dyes and quenchers:
◆ = Orderable online: FAM, VIC, TET, NED, ABY, JUN, Cy5, and Cy5.5 dyes with MGB, TAMRA, QSY, or QSY2 quenchers.
◇ = Available via our Specialty Oligo service
5' dye |
Supported quenchers |
||||
MGB-NFQ |
QSY |
QSY2* |
TAMRA |
||
Dye/channel 1 |
FAM | ◆ | ◆ | ◆ | |
| Dye/channel 2 | VIC | ◆ | ◆ | ◆ | |
| HEX | ◇ | ◇ | |||
| TET | ◆ | ◇ | ◆ | ||
| Yakima Yellow | ◇ | ◇ | |||
| Dye/channel 3 | ABY | ◆ | ◆ | ||
| Cy3 | ◇ | ◇ | |||
| NED | ◆ | ◇ | |||
| PET | ◇ | ◇ | |||
| Dye/channel 4 | JUN | ◆ | ◆ | ||
| ROX | ◇ | ◇ | |||
| Texas Red | ◇ | ◇ | |||
| Alexa 594 | ◇ | ◇ | |||
| Dye/channel 5 | Cy5 | ◆ | ◆ | ||
| Alexa 647 | ◇** | ◇ | |||
| LIZ | ◇ | ◇ | |||
| Dye/channel 6 | Cy5.5 | ◆ | |||
| Alexa 680 | ◇ | ||||
*QSY2 has a longer turnaround time.
**Alexa 647-MGB probes have not been validated. Customers should conduct preliminary studies to confirm suitability for qPCR requirements.
Our Specialty TaqMan Assays and Oligo Service supports a range of custom configurations to meet specific experimental requirements, including:
See the tables below for specialty oligos, assays, scale and format information. Choosing one of the following standard concentrations and reactions will help ensure faster turnaround time for quotes.
Specialty oligos |
Delivery yield (standard concentration)a |
Format |
| Unlabeled primers | Small – 10 nmol | Dry or liquid |
| Medium – 80 nmol | ||
| Large – 130 nmol | ||
| Labeled primers | Small – 10 nmol | |
| Medium – 80 nmol | ||
| Large – 300 nmol | ||
| Probes | Small – 6 nmol | |
| Medium – 20 nmol | ||
| Large – 50 nmol |
Specialty assays |
Size/reactionsa |
Format |
2 primers, 1 probe (gene expression, copy number variation, advanced miRNA assays) |
Small – 360 μL 20X | Liquid |
| Medium – 750 μL 20X | ||
| Large - 967 μL 60X | ||
2 primers, 2 probes (SNP genotyping assays) |
Small – 187 μL 40X | Liquid |
| Medium – 625 μL 40x | ||
| Large - 750 μL 80X | ||
2 primers, RT sequence, 1 probe (legacy miRNA assays)
|
Small – 150 μL 20X | Liquid |
| Medium – 750 μL 20X | ||
| Large - 967 μL 60X | ||
ASP sequence, ASB sequence, LST sequence, LSP sequence (CastPCR mutation detection assays) |
Small – 150 μL 20X | Liquid |
| Medium – 200 μL 20X | ||
| Large - 300 uL 60X |
a: Sequences are available in custom large-scale order.
b: ASP = allele specific primer, ASB = allele specific blocker, LST = locus specific target, LSP = locus specific primer
Specialty assays and oligos are developed under robust quality control procedures designed to help ensure accuracy, consistency, and performance. With multiple customization options, you can tailor assays and oligos to your specific research needs, from custom dye-quencher pairings to optimized concentrations and formulations. Designed for consistent and reproducible results, our solutions help provide the reliability and precision required to advance molecular biology workflows with confidence.
For additional support on our Specialty TaqMan Assay and Oligo Service, email Specialty_Oligos@thermofisher.com.
For Research Use Only. Not for use in diagnostic procedures.