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Protein gel electrophoresis and western blotting are techniques for separating, detecting, and analyzing proteins across a wide range of research applications. Explore product categories for protein separation and detection to find solutions aligned with your research needs and application focus.
Thermo Fisher Scientific offers a comprehensive portfolio of Invitrogen reagents, instruments, and consumables for protein gel electrophoresis and western blotting. Designed for compatibility and consistent performance, these solutions support reliable results across protein analysis applications.
Fast western blot workflow with iWestern solutions
Blot in hours, not days with an integrated workflow designed to reduce hands-on time and variability.
Foundational resources and tools to advance your skills
Access foundational guides and enablement resources that support protein analysis workflows, including gel electrophoresis and western blotting.
Protein gel electrophoresis handbook
Explore key principles, gel formats, and workflow considerations to support reliable protein separation and publication-quality results.
Protein gel guide
Compare protein gel chemistries, understand their performance differences, and identify recommended protein ladders to support confident gel selection.
Western blotting handbook
Review workflow fundamentals, detection strategies, and optimization considerations to support consistent, high-quality western blot results.
Protein Research Essentials Sampling Program
Evaluate select western blotting and protein analysis reagents to identify solutions that fit your research needs—before you buy!
Related pages
Related applications
The following are common steps and applications associated with protein analysis workflows.
Research areas
Protein electrophoresis and western blotting support a wide range of research areas. Explore these research areas to learn how protein analysis techniques support discovery across disciplines.
Frequently asked questions
Gel electrophoresis separates proteins by size or charge, while western blotting transfers those separated proteins to a membrane for specific detection using antibodies. Together, they allow researchers to separate, identify, and analyze target proteins.
No, a western blot is not always required after electrophoresis. Gel electrophoresis alone can be used to assess protein size, purity, or overall expression patterns. A western blot is performed when specific identification or detection of a target protein is needed.
The main steps in a western blot are protein separation, transfer to a membrane, blocking, antibody detection, and imaging. Proteins are first separated on a gel, then transferred to a membrane where specific antibodies detect the target protein. The signal is captured using a detection and imaging system.
Protein assays are typically performed before gel electrophoresis to quantitate total protein concentration. Accurate quantification helps ensure equal sample concentration loading which improves consistency in downstream protein analysis workflows.
Educational content and support
Access protocols, reference materials, and technical support to help support protein analysis workflows.
For Research Use Only. Not for use in diagnostic procedures.