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Infographic: AAV Polishing for Scalable Full Capsid Enrichment

From resin screening to scale-up: Developing an AEX workflow for AAV purification

Empty, partial, and full AAV capsids have similar physicochemical properties, making full/empty capsid separation a challenge during AAV purification. Anion exchange chromatography offers a scalable polishing approach, but method development requires careful optimization for each serotype, payload, and process.

 

This infographic presents practical considerations for building an AEX polishing workflow, from sample preparation and resin screening through buffer optimization, elution development, method confirmation, and scale-up.

Explore key considerations for AAV polishing

  • Addressing recovery, purity, scalability, and serotype variability
  • Screening resins using linear gradients
  • Optimizing pH, buffer, salts, excipients, loading, and flow rate
  • Transferring an optimized method to scalable column formats

Not sure which resin is right for you?

The Bioprocess Resin Selection Guide is an interactive guide that helps scientists evaluate chromatography resins based on molecule type, application, and scale. Identify products that meet your purification goals and process needs by filtering data on resin chemistry, capacity, and operating conditions.
 

For research and development use and further manufacturing in support of FDA-regulated end uses.  Not for diagnostic use or direct administration to humans or animals.