Markers for Characterizing Neural Subtypes

After cells are isolated from tissue or differentiated from pluripotent precursors, the resulting population needs to be characterized to confirm whether the target population has been obtained. This chapter lists cell-type specific antibody markers commonly used for immunocytochemical (ICC) and flow cytometric analysis of neural subtypes.


Cell-type Specific Antibodies for Characterizing Neural Subtypes

Cell type	Antigen	Type	ICC dilution*
Neural stem cells	Sox1	Goat IgG	10 μg/mL
	Sox2	Mouse IgG	2 μg/mL
	Nestin	Mouse IgG	1:500
	CD133	Rabbit IgG	1:100
Neuronal progenitors Neurons (Pan)	MAP2	Mouse IgG	1:200
	HuC/Dl	Mouse IgG	10 μg/mL
	NF	Mouse IgG	1:100
	NCAM	Mouse IgG	1:50
	βIII tubulin	Mouse IgG	1:2,000
	Dcx	Rabbit IgG	1:200
Dopaminergic neurons	TH	Rabbit IgG	1:1,000
Motoneurons	Isl1	Mouse IgG	1:50
	HB9	Mouse IgG	1:50
GABAergic/Glutaminergic neurons	GABA	Rabbit IgG	1:2,000
Cholinergic neurons	ChAT	Goat IgG	1:100
Astrocyte progenitors Astrocytes	CD44	Mouse IgG	1:50
	GFAP	Rat IgG	1:100
	GFAP	Rabbit IgG	1:200
Oligodendrocyte progenitors Oligodendrocytes	GalC	Mouse IgG	1:200
	NG2	Mouse IgG	1:200
	A2B5	Mouse IgM	1:2,000
	O4	Mouse IgM	1:50
Proliferation	Ki67	Rabbit IgG	1:50
	EdU	Chemicals	1:1,000
Isotype control	Mouse	IgM and IgG	Use as is
	Rabbit	IgG	Use as is

* These are recommended starting concentrations for ICC applications; optimal working concentrations must be determined empirically.