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Invitrogen
Human CDC7/DBF4, GST Tag Recombinant Protein
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产品信息
PV6276
产品规格
种属
Human
Expression System
Baculovirus
氨基酸序列
1-574
标签
GST-tag
分子量
91.2-103.5 kDa
分类
Recombinant
类型
Protein
偶联物
形式
Liquid
浓度
See Label
纯化类型
purified
保存液
50mM tris HCl, pH 7.5, with 10mM glutathione, 25% glycerol, 0.1mM EDTA, 0.1mM PMSF, 150mM NaCl, 0.25mM DTT
内含物
no preservative
保存条件
-80°C, Avoid Freeze/Thaw Cycles
运输条件
Dry ice
产品详细信息
For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 µg, 10 µg and 20 µg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 µL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase.
Recombinant full-length human CDC7 and DBF4 proteins were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
• Validated in LanthaScreen™ Kinase Binding Assay
• Protocols available for simple use and optimized performance
• Multiple pack sizes available for convenient use
Related Product:
LanthaScreen™ Eu Kinase Binding Assay
Protein Form: Recombinant
The gene accession number for the subunits (CD7/DBF4) are NP_003494.1 and NP_006707.1.
靶标信息
CDC7, or Cell Division Cycle 7, is a serine/threonine kinase that plays a crucial role in the initiation of DNA replication. It is essential for the activation of replication origins during the S phase of the cell cycle.
ASK, also known as Activator of S Phase Kinase/DBF4, is a regulatory subunit that binds to CDC7 and activates its kinase activity. The binding of ASK to CDC7 is necessary for the proper functioning of CDC7, as it regulates the activation and specificity of the kinase.
Together, the CDC7/ASK complex is critical for the regulation of DNA replication. This complex phosphorylates components of the pre-replication complex at replication origins, thereby promoting the initiation of DNA synthesis. By ensuring that replication origins fire at the appropriate time, the CDC7/ASK complex helps to maintain genomic stability and proper cell cycle progression.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Activator of S phase kinase; CDC7 (cell division cycle 7, S. cerevisiae, homolog)-like 1; CDC7-related kinase; cell division cycle 7 homolog; cell division cycle 7-like protein 1; Cell division cycle 7-related protein kinase; Chiffon homolog A; DBF4 homolog; DBF4 zinc finger A; DBF4-type zinc finger-containing protein 1; Protein DBF4 homolog A; zinc finger, DBF-type containing 1
基因别名: ASK; CDC7; CDC7L1; CHIF; DBF4; DBF4A; HsCDC7; Hsk1; huCDC7; ZDBF1
UniProt ID: (Human) O00311, (Human) Q9UBU7
Entrez Gene ID: (Human) 8317, (Human) 10926
