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Invitrogen
Mouse IL-3 Recombinant Protein
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产品信息
34-8031-82
产品规格
Expression System
E. coli
分类
Recombinant
类型
Protein
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
0.5 mg/mL
Amount
100 µg
保存条件
-80°C
运输条件
Dry ice
产品详细信息
Description: Mouse IL-3, also called Mast Cell Growth Factor (MCGF), Multi-Colony Stimulating Factor (multi-CSF), Eosinophil-CSF (E-CSF), is produced by activated T cells, mast cells and eosinophils. IL-3 is a hematopoietic growth factor that stimulates colony formation of erythroid, megakaryocyte, neutrophil, eosinophil, basophil, mast cell and monocytic lineages. Most of these functions are enhanced or dependent on co-stimulation with other cytokines.
Applications Reported: Recombinant mouse IL-3 is biologically active.
Applications Tested: Measured by mouse MC/9 cell proliferation assay. The ED50 is 0.3 ng/mL, corresponding to a specific activity of 3.3 x 10e6 Units/mg.
Source: E. coli expressed amino acids Ala 27-Cys 166 of mature mouse IL-3 accession # NM_010556.
Bioactivity: Measured by mouse MC/9 cell proliferation assay. The ED50 is 0.3 ng/mL, corresponding to a specific activity of 3.3 x 10e6 Units/mg.
Endotoxin: Less than 0.01 ng/ug cytokine as determined by the LAL assay. Purity: >98% as determined by SDS-PAGE.
Molecular Weight: The protein may be methionylated at the N-terminal and without the met has a predicted molecular mass of 15,673. The DTT reduced protein migrates as an 16 kDa polypeptide on SDS-PAGE. The non-reduced protein migrates as a 14 kDa polypeptide.
Storage and handling: For best recovery, quick-spin vial prior to opening. Use in a sterile environment.
靶标信息
Interleukin 3 (IL-3), also called Mast Cell Growth Factor (MCGF), Multi-Colony Stimulating Factor (multi-CSF), or Eosinophil-CSF (E-CSF), is produced by activated T cells, mast cells and eosinophils. IL-3 is a hematopoietic growth factor that stimulates colony formation of erythroid, megakaryocyte, neutrophil, eosinophil, basophil, mast cell and monocytic lineages. Most of these functions are enhanced or dependent on co-stimulation with other cytokines. Human and mouse IL-3 share only 29% sequence identity at the amino acid level. The bioactivity of IL-3 is highly species specific. For example, human IL-3 has no activity on mouse cells. IL-3 is a potent growth promoting cytokine. IL-3 is capable of supporting the proliferation of a broad range of hematopoietic cell types, and is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. IL-3 has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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