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Invitrogen
Mouse TRANCE (RANKL) Recombinant Protein
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Product Details
14-8612-62
Product Specifications
Expression System
E. coli
Class
Recombinant
Type
Protein
Conjugate
Form
Liquid
Concentration
0.1 mg/mL
Storage conditions
-80°C
Shipping conditions
Dry ice
Product Specific Information
Description: RANK Ligand (Receptor Activator for Nuclear Factor kappaB Ligand), also known as TNF-related activation-induced cytokine (TRANCE), along with RANK are members of the TNF superfamily. RANK-L can be found as a soluble protein expressed by activated T lymphocytes, which plays a role in dendritic cell maturation. In addition it has also been shown to be cleaved by TACE (TNF-alpha convertase) releasing an ectodomain variant of the cell bound form. Lastly RANK-L is expressed as a Type II transmembrane protein expressed by osteoblast lineages. The receptor RANK, located on osteoclastic and dendritic cells can recognize all forms of RANK-L. RANK-L plays a critical role in bone development and mineralization. High levels of RANK-L have been associated with degenerative bone diseases.
Applications Reported: Recombinant mouse RANK-L is biologically active.
Applications Tested: ED50, as measured by induction of osteoclast formation on mouse RAW264.7 cells, is 4.8 ng/mL.
Source: E. coli expressed amino acid Lys158-Asp316 accession # Q3TWY5.
Bioactivity: The ED50, as measured by induction of osteoclast formation on mouse RAW264.7 cells, is 4.8 ng/mL.
Endotoxin: Less than 0.01 ng/ug cytokine as determined by the LAL assay. Purity: >90% as determined by reducing SDS-PAGE.
Molecular Weight: 20 kDa.
Storage and handling: For best recovery, quick-spin vial prior to opening. Use in a sterile environment.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. Two alternatively spliced transcript variants have been found.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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