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Gibco
PeproGMP® Human EGF Recombinant Protein, PeproTech®
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PeproGMP® Human EGF Protein (GMP100-15-100UG) in Bioactivity
Cell proliferation assay analysis of PeproGMP® Human EGF Recombinant Protein, PeproTech (Product # GMP100-15-100UG) compared to Human EGF, Animal-Free Recombinant Protein, PeproTech (Product # AF-100-15-1MG), demonstrating comparable bioactivity across product grades.
产品信息
GMP100-15-100UG
应用
建议稀释比
已发表文章
产品规格
种属
Human
已发表种属
Human,
Mouse
Expression System
E. coli
氨基酸序列
NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW ELR
分子量
6.2 kDa
分类
Recombinant
类型
Protein
纯度
≥ 98% by SDS-PAGE gel and HPLC analyses.
内毒素浓度
≤0.1 EU/µg
活性
Determined by the proliferation of mouse Balb/c 3T3 cells. PeproGMP Recombinant Human EGF has been tested against the WHO standard NIBSC 91/530. Find the International Unit (IU) value here.
偶联物
形式
Lyophilized
纯化类型
purified
内含物
no preservative
保存条件
-20°C or -80°C if preferred
运输条件
Ambient
产品详细信息
The EGF protein is a potent growth factor that stimulates the proliferation of various epiderminal and epithelial cells. The PeproTech Recombinant Human EGF is a 6.2 kDa globular protein containing 53 amino acid residues including 3 intramolecular disulfide bonds.
Reconstitute using sterile water (i.e., WFI) to a concentration of 0.1-1.0 mg/ml.
Additional product information, specifications, handling and reconstitution information are provided on the Certificate of Analysis.
All PeproGMP® products are manufactured using animal-derived component free materials.
Product Description/Intended Use: PeproTech GMP recombinant proteins are manufactured for use as ancillary materials by applying applicable principles of GMP and quality control requirements from USP (United States Pharmacopeia) Chapter <1043> Ancillary Materials for Cell, Gene, and Tissue-Engineered Products.
靶标信息
EGF (Epidermal growth factor) exerts its actions by binding to the EGF Receptor, a 170 kDa protein kinase. Activation of EGFR initiates diverse cellular pathways in response to toxic environmental stimuli, or to EGF binding to the receptor, the EGFR forms homo- or heterodimers with other family members. Each dimeric receptor complex initiates a distinct signaling pathway by recruiting different Src homology 2 (SH2) containing effector proteins. EGF is far and wide expressed in kidney, cerebrum, prostrate and salivary glands. EGF acts as a potent mitogenic factor and the phosphorylated receptor recruits adapter proteins like GRB2 that activates complex downstream signaling cascades. EGF activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STAT modules. Research studies suggest the protein may also play important role in activating the NF-kappa-B signaling cascade. Defects in the EGGF gene are the cause of hypomagnesemia type 4 and dysregulation has been associated with the growth and progression of certain cancers.
For research use or further manufacturing. Not for diagnostic use or direct administration into humans or animals.
生物信息学
蛋白别名: beta-urogastrone; EGF; Epidermal growth factor; H-EGF; Pro-epidermal growth factor
基因别名: EGF; HOMG4; URG
UniProt ID: (Human) P01133
Entrez Gene ID: (Human) 1950
