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Invitrogen
14-3-3 gamma Polyclonal Antibody
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14-3-3 gamma Antibody (PA1-86556) in WB
Western blot analysis of 14-3-3 isoforms in HEK293 cell extracts using a 14-3-3 gamma polyclonal antibody (Product # PA1-86556) (lane 1), a zeta 14-3-3 (lane 2), a C-epsilon-14-3-3 (lane 3), a N-epsilon-14-3-3 (Lane 4), a eta 14-3-3 (Lane 5). The 14-3-3 isoforms run at 30kDa except epsilon 14-3-3.
产品信息
PA1-86556
应用
建议稀释比
已发表文章
产品规格
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptide of acetylated N-terminal amino acids 1-10 of sheep. Peptide sequence: Ac. V D R E Q L V Q K A C of sheep 14-3-3 gamma.
偶联物
形式
Liquid
保存条件
4°C or -20°C if preferred
运输条件
Wet ice
RRID
产品详细信息
Predicted to react with all mammals based on sequence homology.
Heat-mediated antigen retrieval is recommended for the staining of paraffin sections. A suggested positive control for immunohistochemical applications is normal brain tissue. In Western blot, this antibody detects a band at ~30kDa in HEK293 cell lysate.
靶标信息
14-3-3 gamma encodes a product that belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the rat ortholog. It is induced by growth factors in human vascular smooth muscle cells, and is also highly expressed in skeletal and heart muscles, suggesting an important role for this protein in muscle tissue. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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