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Invitrogen
14-3-3 sigma Polyclonal Antibody
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14-3-3 sigma Antibody (PA5-95056) in ICC/IF
Immunocytochemistry analysis of SFN using anti-SFN antibody (Product # PA5-95056) . SFN was detected in a section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-SFN antibody (Product # PA5-95056) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA5-95056
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthetic peptide corresponding to a sequence in the middle region of human 14-3-3 sigma (140-156aa KKRIIDSARSAYQEAMD).
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
浓度
500 µg/mL
规格
100 µg
纯化类型
Affinity chromatography
保存液
PBS with 4mg trehalose
内含物
no preservative
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human Hela whole cell, human PC-3 whole cell, rat brain tissue, mouse brain tissue. IHC: human intestinal cancer tissue, human mammary cancer tissue, human oesophagus squama cancer tissue. ICC/IF: U20S cell, U20S cell. Flow: A431 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
靶标信息
This gene encodes a cell cycle checkpoint protein. The encoded protein binds to translation and initiation factors and functions as a regulator of mitotic translation. In response to DNA damage this protein plays a role in preventing DNA errors during mitosis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: 14-3-3 protein sigma; 14-3-3 sigma protein; 14-3-3sigma; HGNC:10773; Stratifin; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, sigma polypeptide
基因别名: Er; Mkrn3; Mme1; Sfn; Ywhas
UniProt ID: (Mouse) O70456
Entrez Gene ID: (Mouse) 55948, (Rat) 313017
regulation of cyclin-dependent protein serine/threonine kinase activity
release of cytochrome c from mitochondria
keratinocyte development
intrinsic apoptotic signaling pathway in response to DNA damage
regulation of epidermal cell division
negative regulation of keratinocyte proliferation
keratinocyte differentiation
positive regulation of cell growth
keratinization
negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
skin development
positive regulation of epidermal cell differentiation
positive regulation of protein export from nucleus
regulation of cell cycle
establishment of skin barrier
biological_process
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Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
