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Invitrogen
53BP1 Recombinant Polyclonal Antibody (7HCLC)
高级验证
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
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53BP1 Antibody (712578) in ICC/IF
Knockdown of 53BP1 was achieved by transfecting HeLa cells with specific siRNA (Silencer® select Product # s14314, s14315). Immunofluorescence analysis was performed on HeLa cells (untransfected, panel a-d), transfected with 53BP1 specific siRNA (panel i-l) or non-specific scrambled siRNA (panels e-h). Cells were fixed, permeabilized, and labeled with Anti-53BP1 Recombinant Rabbit Polynoclonal Antibody (Product # 712578, 1:100 dilution), followed by Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjug... View More
产品信息
712578
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
Expression System
Expi293
分类
Recombinant Polyclonal
类型
Antibody
克隆号
7HCLC
抗原
Protein corresponding to human 53BP1 [aa241-aa440]
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.4
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2848248
产品详细信息
This antibody is predicted to react with Mouse, Rat, Bovine.
Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.
靶标信息
53BP1 (P53-binding protein 1) plays a critical role in tumor suppression and is a putative substrate of ATM kinase. Upon DNA damage, 53BP1 is phosphorylated and localizes to the presumptive sites of damage, specifically, double-strand breaks. 53BP1 may have a role in checkpoint signaling during mitosis, enhances TP53-mediated transcriptional activation, and participates in DNA repair by maintaining genomic stability.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: 53BP1; FLJ41424; MGC138366; p53-binding protein 1; TP53-binding protein 1; tumor protein 53-binding protein, 1; tumor protein p53-binding protein, 1
基因别名: 53BP1; p202; p53BP1; TDRD30; TP53; TP53BP1
UniProt ID: (Human) Q12888
Entrez Gene ID: (Human) 7158
DNA damage checkpoint
double-strand break repair via nonhomologous end joining
transcription, DNA-templated
cellular response to DNA damage stimulus
protein sumoylation
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of sequence-specific DNA binding transcription factor activity
cellular response to X-ray
