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Invitrogen
6x-His Tag Monoclonal Antibody (AD1.1.10), HRP
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6x-His Tag Antibody (MA1-80218) in WB
Western Blot was performed using Anti-6x-His Tag Monoclonal Antibody (AD1.1.10), HRP (Product # MA1-80218) by loading whole cell extracts of untransfected and transiently transfected HEK-293E lysates: untransfected, 60 µg (Lane 1), empty vector control, 60 µg (Lane 2), V5-H3-His, 60 µg (Lane 3), His-p65-YFP, 60 µg (Lane 4), His-p65-YFP, 40 µg (Lane 5), His-p65-YFP, 20 µg (Lane 6) and 25 ng Positope (Lane 7) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a ni... View More
产品信息
MA1-80218
应用
建议稀释比
已发表文章
产品规格
种属反应
Tag
已发表种属
C. elegans,
Tag
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
AD1.1.10
抗原
PAX6 transcription factor linked to histidine tag.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.4
内含物
0.0095% Methylisothiazolone
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Mouse anti Histidine tag antibody, clone AD1.1.10, recognizes proteins and peptides containing the motif H-H-H-H-H-H and is therefore of value in detecting proteins containing histidine tags.
靶标信息
The 6x His tag is a synthetic oligo peptide consisting of 6 consecutive histidine residues (HHHHHH). The His tag is commonly expressed as a tag at either N-or C-terminal regions of recombinant proteins to allow isolation or purification by immobilized metal affinity chromatography. Epitope tagging is a technique in which a known epitope is fused to a recombinant protein using genetic engineering. By choosing a particular epitope and recombinant protein combination, epitope tagging makes it possible to detect proteins for which no antibody is available. His epitope tagged proteins that contain a stretch of histidine residues at the carboxyl or amino terminus are used for purification of recombinant proteins by means of metal chelate chromatography due to metal binding properties of histidine clusters on His tagged proteins. For example, the affinity of the histidine-tag motif to Ni2+ by chelation is strong and selective enough to enable purification of the protein to homogeneity by affinity chromatography on a Ni2+-NTA adsorbent. His tag-specific antibodies are used to facilitate detection or coimmunopreciptation of tagged proteins.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
