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Agrisera
A12,2/RNA polymerase I subunit (homolog of Pol II Rpb9) Polyclonal Antibody
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A12,2/RNA polymerase I subunit (homolog of Pol II Rpb9) Antibody (AS07-225) in WB
10 µg of total protein from (1) Arabidopsis thaliana and (4) Oryza sativa leaves extracted with PEB, AS08 300, as well as (2) cytosolic and (3) nuclear fractions of Arabidopsis thaliana leaves were separated on 4-12% Nu PAGE(Invitrogen) LDS-PAGE and blotted 1 h to nitrocellulose. Filters were blocked 1 h with 2% low-fat milk powder in TBS-T (0.1% TWEEN 20) and probed with anti-A 12.2 (AS07 255,1:1,000 1 h) ... View More
产品信息
AS07-225
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated peptide derived from the Arabidopsis thaliana A12.2 (At3g25940) protein sequence.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
规格
200 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 143 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana
靶标信息
POLR2I is a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with two other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. POLR2I has two zinc finger motifs with conserved cysteines and the subunit does possess zinc binding activity. Western blots using two different antibodies against two unique regions of this protein target confirm the same apparent molecular weight in our tests. This gene encodes a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with two other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. The product of this gene has two zinc finger motifs with conserved cysteines and the subunit does possess zinc binding activity.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: hRPB14.5; RPB9
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