Invitrogen

ACTBL2 Polyclonal Antibody

查看其他2个ACTBL2抗体

问题与答案

问题与答案

问题与答案

抗体检测数据 (3)

ACTBL2 Antibody in Immunocytochemistry (ICC/IF)

ACTBL2 Antibody in Immunocytochemistry (ICC/IF)

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ACTBL2 Antibody (PA5-102030) in ICC/IF

Immunocytochemistry analysis of ACTBL2 in Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with ACTBL2 polyclonal antibody (Product # PA5-102030) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).

ACTBL2 Antibody in Immunocytochemistry (ICC/IF)

ACTBL2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ACTBL2 Antibody in Western Blot (WB)

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ACTBL2 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of ACTBL2 in Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with ACTBL2 polyclonal antibody (Product # PA5-102030) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).

ACTBL2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of ACTBL2 in rat muscle tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with ACTBL2 polyclonal antibody (Product # PA5-102030) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.

ACTBL2 Antibody in Western Blot (WB) — Western blot analysis of ACTBL2 in HepG2. Samples were incubated with ACTBL2 polyclonal antibody (Product # PA5-102030).

Immunogen image

ACTBL2 Polyclonal Antibody

Immunogen Image ACTBL2 Polyclonal Antibody

产品信息

PA5-102030

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:1,000

-

免疫组化（石蜡） (IHC (P))

1:50-1:200

-

免疫细胞化学 (ICC/IF)

1:100-1:500

-

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human ACTBL2(Accession Q562R1), corresponding to amino acid residues H89-A139.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Antibody detects endogenous levels of total ACTBL2.

靶标信息

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: actin, beta-like 2; actin-like protein; Beta-actin-like protein 2; Kappa-actin

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基因别名: 4732495G21Rik; ACT; ACTBL2

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UniProt ID: (Human) Q562R1, (Mouse) Q8BFZ3

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Entrez Gene ID: (Human) 345651, (Mouse) 238880

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molecular_function ATP binding nucleotide binding actin and actin related protein actin or actin-binding cytoskeletal protein cytoskeletal protein

biological_process