Invitrogen

ADFP Polyclonal Antibody

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ADFP Antibody in Immunohistochemistry (Frozen) (IHC (F)) — Immunohistochemical analysis of ADFP in mouse liver. Samples were incubated with ADFP polyclonal antibody (Product # PA1-16972) followed by using DAB with hematoxylin counterstain.

ADFP Antibody in Western Blot (WB) — Western blot analysis of ADFP in 0.5 mg/mL HepG2 lysate. Samples were incubated in ADFP polyclonal (Product # PA1-16972). This experiment was performed under reducing conditions using the 12-230 kDa separation system.

ADFP Antibody in Immunocytochemistry (ICC/IF) — Fig. 5 CCTbeta3 in MEFs. a Distribution of mCherry-tagged CCT isoforms (magenta) and LDs (green) in MEFs starved for 8 h. Bar, 10 mum. b Distribution of CCTbeta3-mCherry (magenta) and perilipin-2 (green) in MEFs. Bars, 10 mum. (a) Regular medium containing OA for 2 days. (b) Regular medium containing OA for 2 days and then in starvation medium for 1 h. (c) Starvation medium containing OA for 8 h. c Western blotting of the total cell lysate and the LD fraction. MEFs overexpressing CCTbeta3 were cultured in regular medium containing OA for 8 h (OA) or in starvation medium containing OA for 8 h (EBSS). Twenty micrograms (total cell) and one microgram (LDs) of proteins were loaded. d , e The effect of combined DGAT1 and ACAT inhibition on the autophagic flux and the isolation membrane/autophagosome. Cells were cultured for 8 h in starvation medium, with or without a mixture of 20 muM T863 and 1 muM avasimibe for the last 2 h. d Autophagic flux. A representative result of three independent experiments. e Immunolabeling of endogenous LC3. Bar, 10 mum. The total area of LC3 puncta in a cell was compared. The center line indicates the median, box boundaries indicate the 25th and 75th percentiles, whiskers are Tukey-type, and the average is marked as +. Pooled data obtained in three independent experiments ( n = 90); **** p < 0.0001 (two-tailed Mann-Whitney test). f The effect of CQ in MEFs cultured in starvation medium for either 2 or 8 h. CQ (20 muM) was added to the medium for the

ADFP Antibody in Immunohistochemistry (IHC) — Figure 2 Analyses of mammary gland on lactation day 2 ( A ) Morphology of mammary glands on lactation day 2. The fourth mammary glands were dissected. Milk in the mammary glands of mutant mice was visible (arrowhead). ( B ) Reduced epithelial density of mammary glands of mutant mice on lactation day 2 (Hematoxylin and Eosin staining). ( C ) Milk content was not observed in the mammary gland ducts of mutant mice. Milk contents were marked by #. Ducts in mutant mice without milk contents marked by *. ( D ) Immunostaining for the milk protein, PLIN2.

产品信息

PA1-16972

应用	建议稀释比	已发表文章
免疫印迹 (WB)	2 µg/mL	查看1篇已发表文章 1篇已发表文章
免疫组化 (IHC)	-	查看2篇已发表文章 2篇已发表文章
免疫组化（石蜡） (IHC (P))	1:200	查看1篇已发表文章 1篇已发表文章
免疫组化（冰冻） (IHC (F))	Assay-Dependent	-
免疫细胞化学 (ICC/IF)	1:50-1:200	查看2篇已发表文章 2篇已发表文章

产品规格
种属反应	Bovine, Human, Mouse, Rat
已发表种属	Mouse
宿主/亚型	Rabbit / IgG
分类	Polyclonal
类型	Antibody
抗原	Synthetic peptide corresponding to the C-terminal region (within residues 350-425) of mouse ADFP.
偶联物	Unconjugated
形式	Liquid
浓度	1 mg/mL
纯化类型	Antigen affinity chromatography
保存液	PBS
内含物	0.02% sodium azide
保存条件	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2223607

产品详细信息

The target sequence has 88% sequence homology with rat ADFP.

靶标信息

Milk lipid globules from humans, cows and rats contain a protein identified as adipocyte differentiation-related protein (ADFP). It is associated with the globule surface membrane material. This protein, previously believed to be specific to adipocytes, is a major constituent of the globule surface and is present in a detergent-insoluble complex that contains butyrophilin and xanthine oxidase. ADFP (Adipophilin) is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells. In tissues, however, expression of adipophilin is restricted to specific cell types, such as lactating mammary epithelial cells, adrenal cortex cells, Sertoli and Leydig cells of the male reproductive system, and steatosis or fatty change hepatocytes in alcoholic liver cirrhosis. ADFP may be a possible new marker for the identification of specialized differentiated cells containing lipid droplets and for diseases associated with fat-accumulating cells.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Mex3c mutation affects lactation through impairing milk ejection in female mice.

Bioscience reports

Du Y,Sun D,Li Y

PA1-16972 was used in Immunohistochemistry (Paraffin) to study the effect of the Mex3c mutation on lactation in female mice.

Wed Dec 23 00:00:00 EST 2020

Mex3c mutation affects lactation through impairing milk ejection in female mice.

Bioscience reports

Du Y,Sun D,Li Y

PA1-16972 was used in Immunohistochemistry (Paraffin) to study the effect of the Mex3c mutation on lactation in female mice.

Wed Dec 23 00:00:00 EST 2020

Long-term autophagy is sustained by activation of CCTβ3 on lipid droplets.

Nature communications

Ogasawara Y,Cheng J,Tatematsu T,Uchida M,Murase O,Yoshikawa S,Ohsaki Y,Fujimoto T

PA1-16972 was used in Immunocytochemistry-immunoflourescence to demonstrate that phosphatidylcholine synthesis through CCTβ3 activation on lipid droplets is crucial for sustaining autophagy and long-term cell survival.

Tue Sep 08 00:00:00 EDT 2020

Long-term autophagy is sustained by activation of CCTβ3 on lipid droplets.

Nature communications

Ogasawara Y,Cheng J,Tatematsu T,Uchida M,Murase O,Yoshikawa S,Ohsaki Y,Fujimoto T

Tue Sep 08 00:00:00 EDT 2020

Lung developmental arrest caused by PDGF-A deletion: consequences for the adult mouse lung.

American journal of physiology. Lung cellular and molecular physiology

Gouveia L,Kraut S,Hadzic S,Vazquéz-Liébanas E,Kojonazarov B,Wu CY,Veith C,He L,Mermelekas G,Schermuly RT,Weissmann N,Betsholtz C,Andrae J

PA1-16972 was used in Immunocytochemistry to indicate that Pdgfa deletion-induced lung developmental arrest has consequences for the adult lung at the morphological and functional level.

Wed Apr 01 00:00:00 EDT 2020

Myonectin deletion promotes adipose fat storage and reduces liver steatosis.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Little HC,Rodriguez S,Lei X,Tan SY,Stewart AN,Sahagun A,Sarver DC,Wong GW

PA1-16972 was used in Western Blotting to determine myonectin's requirement for metabolic homeostasis in a genetic mouse model.

Mon Jul 01 00:00:00 EDT 2019

PDGF-A signaling is required for secondary alveolar septation and controls epithelial proliferation in the developing lung.

Development (Cambridge, England)

Gouveia L,Betsholtz C,Andrae J

PA1-16972 was used in Immunohistochemistry-immunofluorescence to indicate that PDGF-A signaling is involved in myofibroblast proliferation and migration in the lung.

Tue Apr 10 00:00:00 EDT 2018

生物信息学

蛋白别名: Adipophilin; adipose differentiation related protein; Adipose differentiation-related protein; ADRP; ADRP antibody; MGC10598; Perilipin-2

基因别名: AA407157; ADFP; ADPH; ADRP; PLIN2

UniProt ID: (Human) Q99541, (Bovine) Q9TUM6, (Mouse) P43883

Entrez Gene ID: (Human) 123, (Bovine) 280981, (Mouse) 11520, (Rat) 298199

分子生物学功能: scaffold/adaptor protein

功能

response to organic cyclic compound long-chain fatty acid transport lipid storage response to drug

参与通路

protein binding molecular_function

ADFP Polyclonal Antibody

产品信息

PA1-16972

免疫印迹 (WB)

免疫组化 (IHC)

免疫组化（石蜡） (IHC (P))

免疫组化（冰冻） (IHC (F))

免疫细胞化学 (ICC/IF)

种属反应

已发表种属

宿主/亚型

分类

类型

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

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