Invitrogen

AFG3L2 Polyclonal Antibody

图: 1 / 3

AFG3L2 Antibody (PA5-95347) in WB

Western blot analysis of AFG3L2 in, Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human MDA-MB-453 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat liver tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse liver tissue lysates. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 µg of sample under reducing conditions. Aft... View More

Product Image Gallery

AFG3L2 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of AFG3L2 using anti-AFG3L2 antibody (Product # PA5-95347) . AFG3L2 was detected in a section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-AFG3L2 antibody (Product # PA5-95347) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

AFG3L2 Antibody in Flow Cytometry (Flow) — Flow Cytometry of AFG3L2 in A431 cells (blue line), isotype control rabbit IgG (green line) and unlabeled (red line). Samples were blocked with 10% goat serum, incubated with AFG3L2 Polyclonal Antibody (Product # PA5-95347) at a dilution of 1 μg (per 1x10^6 cells), followed by DyLight®488 conjugated goat anti-rabbit IgG (for 30 minutes at 20°C) using 5-10 μg (per 1x10^6 cells) dilution.

产品信息

PA5-95347

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.5 µg/mL

免疫细胞化学 (ICC/IF)

2 µg/mL

流式细胞分析 (Flow)

1-3 µg/1x10^6 cells

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

E.coli-derived human AFG3L2 recombinant protein (Position: R168-D250).

查看免疫原

偶联物

Unconjugated

形式

Lyophilized

浓度

500 µg/mL

纯化类型

Affinity chromatography

保存液

PBS with 5mg BSA

内含物

0.05mg sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2807150

产品详细信息

Human AFG3L2 shares 100% amino acid (aa) sequence identity with mouse AFG3L2.

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

靶标信息

AFG3L2 is a protein localized in mitochondria and closely related to paraplegin. The paraplegin gene is responsible for an autosomal recessive form of hereditary spastic paraplegia. AFG3L2 gene is a candidate gene for other hereditary spastic paraplegias or neurodegenerative disorders. This gene encodes a protein localized in mitochondria and closely related to paraplegin. The paraplegin gene is responsible for an autosomal recessive form of hereditary spastic paraplegia. This gene is a candidate gene for other hereditary spastic paraplegias or neurodegenerative disorders.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

篇参考文献 (0)

您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。

引用该产品

生物信息学

蛋白别名: AFG3 ATPase family gene 3-like 2; AFG3 ATPase family member 3-like 2; AFG3 like AAA ATPase 2; AFG3(ATPase family gene 3)-like 2; AFG3-like AAA ATPase 2; AFG3-like protein 2; ATPase family gene 3, yeast; FLJ25993; Paraplegin-like protein

基因别名: 2310036I02Rik; AFG3L2; AW260507; Emv66; par; SCA28; SPAX5

UniProt ID: (Human) Q9Y4W6, (Mouse) Q8JZQ2

Entrez Gene ID: (Human) 10939, (Mouse) 69597, (Rat) 307350

分子生物学功能: metalloprotease